A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence

Matthias Jost, Shin Taketa, Martin Mascher, Axel Himmelbach, Takahisa Yuo, Fahimeh Shahinnia, Twan Rutten, Arnis Druka, Thomas Schmutzer, Burkhard Steuernagel, Sebastian Beier, Stefan Taudien, Uwe Scholz, Michele Morgante, Robbie Waugh, Nils Stein

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Inflorescence architecture in small-grain cereals has a direct effect on yield and is an important selection target in breeding for yield improvement. We analyzed the recessivemutation laxatum-a (lax-a) in barley (Hordeum vulgare), which causes pleiotropic changes in spike development, resulting in (1) extended rachis internodes conferring a more relaxed inflorescence, (2) broadened base of the lemma awns, (3) thinner grains that are largely exposed due to reduced marginal growth of the palea and lemma, and (4) and homeotic conversion of lodicules into two stamenoid structures. Map-based cloning enforced by mapping-by-sequencing of the mutant lax-a locus enabled the identification of a homolog of BLADE-ON-PETIOLE1 (BOP1) and BOP2 as the causal gene. Interestingly, the recently identified barley uniculme4 gene also is a BOP1/2 homolog and has been shown to regulate tillering and leaf sheath development. While the Arabidopsis (Arabidopsis thaliana) BOP1 and BOP2 genes act redundantly, the barley genes contribute independent effects in specifying the developmental growth of vegetative and reproductive organs, respectively. Analysis of natural genetic diversity revealed strikingly different haplotype diversity for the two paralogous barley genes, likely affected by the respective genomic environments, since no indication for an active selection process was detected.

Original languageEnglish
Pages (from-to)1113-1127
Number of pages15
JournalPlant Physiology
Volume171
Issue number2
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Inflorescence
Hordeum
internodes
inflorescences
barley
Genes
genes
Arabidopsis
tillering
Growth
Hordeum vulgare
Haplotypes
Breeding
vegetative growth
Organism Cloning
molecular cloning
small cereal grains
haplotypes
Arabidopsis thaliana
genomics

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence. / Jost, Matthias; Taketa, Shin; Mascher, Martin; Himmelbach, Axel; Yuo, Takahisa; Shahinnia, Fahimeh; Rutten, Twan; Druka, Arnis; Schmutzer, Thomas; Steuernagel, Burkhard; Beier, Sebastian; Taudien, Stefan; Scholz, Uwe; Morgante, Michele; Waugh, Robbie; Stein, Nils.

In: Plant Physiology, Vol. 171, No. 2, 01.06.2016, p. 1113-1127.

Research output: Contribution to journalArticle

Jost, M, Taketa, S, Mascher, M, Himmelbach, A, Yuo, T, Shahinnia, F, Rutten, T, Druka, A, Schmutzer, T, Steuernagel, B, Beier, S, Taudien, S, Scholz, U, Morgante, M, Waugh, R & Stein, N 2016, 'A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence', Plant Physiology, vol. 171, no. 2, pp. 1113-1127. https://doi.org/10.1104/pp.16.00124
Jost, Matthias ; Taketa, Shin ; Mascher, Martin ; Himmelbach, Axel ; Yuo, Takahisa ; Shahinnia, Fahimeh ; Rutten, Twan ; Druka, Arnis ; Schmutzer, Thomas ; Steuernagel, Burkhard ; Beier, Sebastian ; Taudien, Stefan ; Scholz, Uwe ; Morgante, Michele ; Waugh, Robbie ; Stein, Nils. / A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence. In: Plant Physiology. 2016 ; Vol. 171, No. 2. pp. 1113-1127.
@article{f3b87f22730e42caa1a018d7a1ae9d67,
title = "A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence",
abstract = "Inflorescence architecture in small-grain cereals has a direct effect on yield and is an important selection target in breeding for yield improvement. We analyzed the recessivemutation laxatum-a (lax-a) in barley (Hordeum vulgare), which causes pleiotropic changes in spike development, resulting in (1) extended rachis internodes conferring a more relaxed inflorescence, (2) broadened base of the lemma awns, (3) thinner grains that are largely exposed due to reduced marginal growth of the palea and lemma, and (4) and homeotic conversion of lodicules into two stamenoid structures. Map-based cloning enforced by mapping-by-sequencing of the mutant lax-a locus enabled the identification of a homolog of BLADE-ON-PETIOLE1 (BOP1) and BOP2 as the causal gene. Interestingly, the recently identified barley uniculme4 gene also is a BOP1/2 homolog and has been shown to regulate tillering and leaf sheath development. While the Arabidopsis (Arabidopsis thaliana) BOP1 and BOP2 genes act redundantly, the barley genes contribute independent effects in specifying the developmental growth of vegetative and reproductive organs, respectively. Analysis of natural genetic diversity revealed strikingly different haplotype diversity for the two paralogous barley genes, likely affected by the respective genomic environments, since no indication for an active selection process was detected.",
author = "Matthias Jost and Shin Taketa and Martin Mascher and Axel Himmelbach and Takahisa Yuo and Fahimeh Shahinnia and Twan Rutten and Arnis Druka and Thomas Schmutzer and Burkhard Steuernagel and Sebastian Beier and Stefan Taudien and Uwe Scholz and Michele Morgante and Robbie Waugh and Nils Stein",
year = "2016",
month = "6",
day = "1",
doi = "10.1104/pp.16.00124",
language = "English",
volume = "171",
pages = "1113--1127",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

TY - JOUR

T1 - A homolog of blade-on-petiole 1 and 2 (BOP1/2) controls internode length and homeotic changes of the barley inflorescence

AU - Jost, Matthias

AU - Taketa, Shin

AU - Mascher, Martin

AU - Himmelbach, Axel

AU - Yuo, Takahisa

AU - Shahinnia, Fahimeh

AU - Rutten, Twan

AU - Druka, Arnis

AU - Schmutzer, Thomas

AU - Steuernagel, Burkhard

AU - Beier, Sebastian

AU - Taudien, Stefan

AU - Scholz, Uwe

AU - Morgante, Michele

AU - Waugh, Robbie

AU - Stein, Nils

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Inflorescence architecture in small-grain cereals has a direct effect on yield and is an important selection target in breeding for yield improvement. We analyzed the recessivemutation laxatum-a (lax-a) in barley (Hordeum vulgare), which causes pleiotropic changes in spike development, resulting in (1) extended rachis internodes conferring a more relaxed inflorescence, (2) broadened base of the lemma awns, (3) thinner grains that are largely exposed due to reduced marginal growth of the palea and lemma, and (4) and homeotic conversion of lodicules into two stamenoid structures. Map-based cloning enforced by mapping-by-sequencing of the mutant lax-a locus enabled the identification of a homolog of BLADE-ON-PETIOLE1 (BOP1) and BOP2 as the causal gene. Interestingly, the recently identified barley uniculme4 gene also is a BOP1/2 homolog and has been shown to regulate tillering and leaf sheath development. While the Arabidopsis (Arabidopsis thaliana) BOP1 and BOP2 genes act redundantly, the barley genes contribute independent effects in specifying the developmental growth of vegetative and reproductive organs, respectively. Analysis of natural genetic diversity revealed strikingly different haplotype diversity for the two paralogous barley genes, likely affected by the respective genomic environments, since no indication for an active selection process was detected.

AB - Inflorescence architecture in small-grain cereals has a direct effect on yield and is an important selection target in breeding for yield improvement. We analyzed the recessivemutation laxatum-a (lax-a) in barley (Hordeum vulgare), which causes pleiotropic changes in spike development, resulting in (1) extended rachis internodes conferring a more relaxed inflorescence, (2) broadened base of the lemma awns, (3) thinner grains that are largely exposed due to reduced marginal growth of the palea and lemma, and (4) and homeotic conversion of lodicules into two stamenoid structures. Map-based cloning enforced by mapping-by-sequencing of the mutant lax-a locus enabled the identification of a homolog of BLADE-ON-PETIOLE1 (BOP1) and BOP2 as the causal gene. Interestingly, the recently identified barley uniculme4 gene also is a BOP1/2 homolog and has been shown to regulate tillering and leaf sheath development. While the Arabidopsis (Arabidopsis thaliana) BOP1 and BOP2 genes act redundantly, the barley genes contribute independent effects in specifying the developmental growth of vegetative and reproductive organs, respectively. Analysis of natural genetic diversity revealed strikingly different haplotype diversity for the two paralogous barley genes, likely affected by the respective genomic environments, since no indication for an active selection process was detected.

UR - http://www.scopus.com/inward/record.url?scp=84973598693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973598693&partnerID=8YFLogxK

U2 - 10.1104/pp.16.00124

DO - 10.1104/pp.16.00124

M3 - Article

VL - 171

SP - 1113

EP - 1127

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -