A histochemical study of acid phosphatases in medullary bone matrix and osteoclasts in laying Japanese quail

Toshio Yamamoto, Hiroshi Nagai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acid phosphatase activity in medullary bone matrix and osteoclasts of laying Japanese quail was examined histochemically. To avoid nonspecific staining, the reactivity of the enzyme was evaluated using both the azo dye method and the lead salt method and nonembedded thick sections and resin-embedded thin sections. The pH of the incubation medium was also varied from the acid range (pH 5.0 and 6.5) to the alkaline range (pH 8.5). Medullary bone osteoclasts contain both tartrate-resistant acid phosphatase (TRAP) activity and fluoride-resistant acid phosphatase (FRAP) activity, and no significant difference in intensity was detected between active and inactive osteoclasts. The entire matrix of medullary bone was positive for tartrate-resistant, fluoride-sensitive acid phosphatase activity. No reaction product was observed in sections incubated in substrate-free and pH 8.5 media. The results demonstrate the existence of FRAP in medullary bone osteoclasts and suggest that medullary bone matrix includes TRAP throughout the matrix.

Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalJournal of Bone and Mineral Research
Volume7
Issue number11
Publication statusPublished - Nov 1992

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Coturnix
Bone Matrix
Osteoclasts
Acid Phosphatase
Fluorides
Azo Compounds
Bone and Bones
Salts
Staining and Labeling
Acids
Enzymes
Tartrate-Resistant Acid Phosphatase

ASJC Scopus subject areas

  • Surgery

Cite this

A histochemical study of acid phosphatases in medullary bone matrix and osteoclasts in laying Japanese quail. / Yamamoto, Toshio; Nagai, Hiroshi.

In: Journal of Bone and Mineral Research, Vol. 7, No. 11, 11.1992, p. 1267-1273.

Research output: Contribution to journalArticle

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