Abstract
The changes in lipid metabolism produced by a coplanar PCB were studied in rats. Male Wistar rats were given a single intraperitoneal injection of 3,3',4,4',5-pentachlorobiphenyl at a dose of 25 mg/kg. After 5 days of administration, total hepatic lipids were treated with 1 M KOH in methanol at 75°C and the liberated fatty acids were analyzed by HPLC after conversion to fluorescent derivatives. In comparison with free-fed and pair-fed control groups, the proportion of arachidonic acid in the PenCB-treated rats was reduced by about 50%, while oleic and linoleic acids increased significantly. We also examined the individual glycerophospholipids, separated by TLC, to see if they were affected by alteration in the fatty acid composition of the whole liver. In all glycerophospholipids, the proportion of arachidonic acid was reduced significantly to the same degree while linoleic acid increased. Changes in the activity of desaturase isozymes have been postulated to explain this unusual lipid metabolism following administration of a toxic PCB and this may contribute to its toxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 99-104 |
| Number of pages | 6 |
| Journal | Toxicology Letters |
| Volume | 91 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 28 1997 |
| Externally published | Yes |
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Keywords
- 3,3'4,4',5-pentachlorobiphenyl
- Arachidonic acid
- Desaturase
- Fatty acid
- Fatty acid composition
- Linoleic acid
- Oleic acid
- PCB
- PenCB
- Polychlorinated biphenyl
ASJC Scopus subject areas
- Toxicology
Cite this
A highly toxic PCB produces unusual changes in the fatty acid composition of rat liver. / Matsusue, Kimihiko; Ishii, Yuji; Ariyoshi, Noritaka; Oguri, Kazuta.
In: Toxicology Letters, Vol. 91, No. 2, 28.04.1997, p. 99-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A highly toxic PCB produces unusual changes in the fatty acid composition of rat liver
AU - Matsusue, Kimihiko
AU - Ishii, Yuji
AU - Ariyoshi, Noritaka
AU - Oguri, Kazuta
PY - 1997/4/28
Y1 - 1997/4/28
N2 - The changes in lipid metabolism produced by a coplanar PCB were studied in rats. Male Wistar rats were given a single intraperitoneal injection of 3,3',4,4',5-pentachlorobiphenyl at a dose of 25 mg/kg. After 5 days of administration, total hepatic lipids were treated with 1 M KOH in methanol at 75°C and the liberated fatty acids were analyzed by HPLC after conversion to fluorescent derivatives. In comparison with free-fed and pair-fed control groups, the proportion of arachidonic acid in the PenCB-treated rats was reduced by about 50%, while oleic and linoleic acids increased significantly. We also examined the individual glycerophospholipids, separated by TLC, to see if they were affected by alteration in the fatty acid composition of the whole liver. In all glycerophospholipids, the proportion of arachidonic acid was reduced significantly to the same degree while linoleic acid increased. Changes in the activity of desaturase isozymes have been postulated to explain this unusual lipid metabolism following administration of a toxic PCB and this may contribute to its toxicity.
AB - The changes in lipid metabolism produced by a coplanar PCB were studied in rats. Male Wistar rats were given a single intraperitoneal injection of 3,3',4,4',5-pentachlorobiphenyl at a dose of 25 mg/kg. After 5 days of administration, total hepatic lipids were treated with 1 M KOH in methanol at 75°C and the liberated fatty acids were analyzed by HPLC after conversion to fluorescent derivatives. In comparison with free-fed and pair-fed control groups, the proportion of arachidonic acid in the PenCB-treated rats was reduced by about 50%, while oleic and linoleic acids increased significantly. We also examined the individual glycerophospholipids, separated by TLC, to see if they were affected by alteration in the fatty acid composition of the whole liver. In all glycerophospholipids, the proportion of arachidonic acid was reduced significantly to the same degree while linoleic acid increased. Changes in the activity of desaturase isozymes have been postulated to explain this unusual lipid metabolism following administration of a toxic PCB and this may contribute to its toxicity.
KW - 3,3'4,4',5-pentachlorobiphenyl
KW - Arachidonic acid
KW - Desaturase
KW - Fatty acid
KW - Fatty acid composition
KW - Linoleic acid
KW - Oleic acid
KW - PCB
KW - PenCB
KW - Polychlorinated biphenyl
UR - http://www.scopus.com/inward/record.url?scp=0031589205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031589205&partnerID=8YFLogxK
U2 - 10.1016/S0378-4274(97)03881-2
DO - 10.1016/S0378-4274(97)03881-2
M3 - Article
C2 - 9175845
AN - SCOPUS:0031589205
VL - 91
SP - 99
EP - 104
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -