A highly toxic PCB produces unusual changes in the fatty acid composition of rat liver

Kimihiko Matsusue, Yuji Ishii, Noritaka Ariyoshi, Kazuta Oguri

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The changes in lipid metabolism produced by a coplanar PCB were studied in rats. Male Wistar rats were given a single intraperitoneal injection of 3,3',4,4',5-pentachlorobiphenyl at a dose of 25 mg/kg. After 5 days of administration, total hepatic lipids were treated with 1 M KOH in methanol at 75°C and the liberated fatty acids were analyzed by HPLC after conversion to fluorescent derivatives. In comparison with free-fed and pair-fed control groups, the proportion of arachidonic acid in the PenCB-treated rats was reduced by about 50%, while oleic and linoleic acids increased significantly. We also examined the individual glycerophospholipids, separated by TLC, to see if they were affected by alteration in the fatty acid composition of the whole liver. In all glycerophospholipids, the proportion of arachidonic acid was reduced significantly to the same degree while linoleic acid increased. Changes in the activity of desaturase isozymes have been postulated to explain this unusual lipid metabolism following administration of a toxic PCB and this may contribute to its toxicity.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalToxicology Letters
Volume91
Issue number2
DOIs
Publication statusPublished - Apr 28 1997
Externally publishedYes

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Glycerophospholipids
3,4,5,3',4'-pentachlorobiphenyl
Poisons
Polychlorinated Biphenyls
Lipid Metabolism
Arachidonic Acid
Liver
Rats
Fatty Acids
Oleic Acids
Linoleic Acids
Linoleic Acid
Intraperitoneal Injections
Chemical analysis
Isoenzymes
Methanol
Wistar Rats
High Pressure Liquid Chromatography
Lipids
Control Groups

Keywords

  • 3,3'4,4',5-pentachlorobiphenyl
  • Arachidonic acid
  • Desaturase
  • Fatty acid
  • Fatty acid composition
  • Linoleic acid
  • Oleic acid
  • PCB
  • PenCB
  • Polychlorinated biphenyl

ASJC Scopus subject areas

  • Toxicology

Cite this

A highly toxic PCB produces unusual changes in the fatty acid composition of rat liver. / Matsusue, Kimihiko; Ishii, Yuji; Ariyoshi, Noritaka; Oguri, Kazuta.

In: Toxicology Letters, Vol. 91, No. 2, 28.04.1997, p. 99-104.

Research output: Contribution to journalArticle

Matsusue, Kimihiko ; Ishii, Yuji ; Ariyoshi, Noritaka ; Oguri, Kazuta. / A highly toxic PCB produces unusual changes in the fatty acid composition of rat liver. In: Toxicology Letters. 1997 ; Vol. 91, No. 2. pp. 99-104.
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