Abstract
We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
| Original language | English |
|---|---|
| Title of host publication | Procedia Engineering |
| Pages | 812-815 |
| Number of pages | 4 |
| Volume | 5 |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
| Event | 24th Eurosensors Conference - Linz, Austria Duration: Sep 5 2010 → Sep 8 2010 |
Other
| Other | 24th Eurosensors Conference |
|---|---|
| Country | Austria |
| City | Linz |
| Period | 9/5/10 → 9/8/10 |
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Keywords
- Biomolecule
- Denaturant
- Electrochemical
- Leakage current microsensor
- Liposome
- Protein
ASJC Scopus subject areas
- Engineering(all)
Cite this
A highly-sensitive leakage current microsensor by using denaturant : detection of target protein by DPPC liposome. / Lorchirachoonkul, P.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Kuboi, R.; Noda, M.
Procedia Engineering. Vol. 5 2010. p. 812-815.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A highly-sensitive leakage current microsensor by using denaturant
T2 - detection of target protein by DPPC liposome
AU - Lorchirachoonkul, P.
AU - Shimanouchi, Toshinori
AU - Yamashita, K.
AU - Umakoshi, H.
AU - Kuboi, R.
AU - Noda, M.
PY - 2010
Y1 - 2010
N2 - We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
AB - We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
KW - Biomolecule
KW - Denaturant
KW - Electrochemical
KW - Leakage current microsensor
KW - Liposome
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=78650624165&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650624165&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2010.09.232
DO - 10.1016/j.proeng.2010.09.232
M3 - Conference contribution
AN - SCOPUS:78650624165
VL - 5
SP - 812
EP - 815
BT - Procedia Engineering
ER -