A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome

P. Lorchirachoonkul, Toshinori Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.

Original languageEnglish
Title of host publicationProcedia Engineering
Pages812-815
Number of pages4
Volume5
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event24th Eurosensors Conference - Linz, Austria
Duration: Sep 5 2010Sep 8 2010

Other

Other24th Eurosensors Conference
CountryAustria
CityLinz
Period9/5/109/8/10

Fingerprint

Microsensors
Liposomes
Leakage currents
Carbonic anhydrase
Proteins
Biomolecules
Conformations
Molten materials

Keywords

  • Biomolecule
  • Denaturant
  • Electrochemical
  • Leakage current microsensor
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Lorchirachoonkul, P., Shimanouchi, T., Yamashita, K., Umakoshi, H., Kuboi, R., & Noda, M. (2010). A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome. In Procedia Engineering (Vol. 5, pp. 812-815) https://doi.org/10.1016/j.proeng.2010.09.232

A highly-sensitive leakage current microsensor by using denaturant : detection of target protein by DPPC liposome. / Lorchirachoonkul, P.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Kuboi, R.; Noda, M.

Procedia Engineering. Vol. 5 2010. p. 812-815.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lorchirachoonkul, P, Shimanouchi, T, Yamashita, K, Umakoshi, H, Kuboi, R & Noda, M 2010, A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome. in Procedia Engineering. vol. 5, pp. 812-815, 24th Eurosensors Conference, Linz, Austria, 9/5/10. https://doi.org/10.1016/j.proeng.2010.09.232
Lorchirachoonkul P, Shimanouchi T, Yamashita K, Umakoshi H, Kuboi R, Noda M. A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome. In Procedia Engineering. Vol. 5. 2010. p. 812-815 https://doi.org/10.1016/j.proeng.2010.09.232
Lorchirachoonkul, P. ; Shimanouchi, Toshinori ; Yamashita, K. ; Umakoshi, H. ; Kuboi, R. ; Noda, M. / A highly-sensitive leakage current microsensor by using denaturant : detection of target protein by DPPC liposome. Procedia Engineering. Vol. 5 2010. pp. 812-815
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