TY - JOUR
T1 - A highly-sensitive leakage current microsensor by using denaturant
T2 - detection of target protein by DPPC liposome
AU - Lorchirachoonkul, P.
AU - Shimanouchi, T.
AU - Yamashita, K.
AU - Umakoshi, H.
AU - Kuboi, R.
AU - Noda, M.
N1 - Funding Information:
This research was supported in part by Grants-in-Aid for Scientific Research (No. 19360162, 21246121, 20760539) from the Japan Society for the Promotion of Science. It was partly supported by the Core University Program between JSPS and VAST.
PY - 2010
Y1 - 2010
N2 - We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
AB - We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
KW - Biomolecule
KW - Denaturant
KW - Electrochemical
KW - Leakage current microsensor
KW - Liposome
KW - Protein
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U2 - 10.1016/j.proeng.2010.09.232
DO - 10.1016/j.proeng.2010.09.232
M3 - Article
AN - SCOPUS:78650624165
VL - 5
SP - 812
EP - 815
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
ER -