A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome

P. Lorchirachoonkul, T. Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states.

Original languageEnglish
Pages (from-to)812-815
Number of pages4
JournalProcedia Engineering
Volume5
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Biomolecule
  • Denaturant
  • Electrochemical
  • Leakage current microsensor
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Engineering(all)

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