A high-throughput chemical screening method for inhibitors and potentiators of hypersensitive cell death using suspension cell culture of Arabidopsis thaliana

Yoshiteru Noutoshi, Ken Shirasu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chemical biology provides an alternative way to identify genes involved in a particular biological process. It has the potential to overcome issues such as redundancy or lethality often found in genetic approaches, since the chemical compounds can simultaneously target all homologous proteins that function at the same step, and chemicals can be applied conditionally. Even with a variety of genetic approaches, the molecular mechanisms of plant hypersensitive cell death that occurs during disease resistance responses remain unclear. Therefore, application of chemical biology should provide new insights into this phenomenon. Here we describe a high-throughput chemical screening procedure to detect hypersensitive cell death quantitatively, using a suspension cell culture of Arabidopsis thaliana and a well-studied avirulent bacterial pathogen, Pseudomonas syringae pv. tomato DC3000 avrRpm1.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages39-47
Number of pages9
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1795
ISSN (Print)1064-3745

Fingerprint

High-Throughput Screening Assays
Arabidopsis
Suspensions
Cell Death
Cell Culture Techniques
Pseudomonas syringae
Biological Phenomena
Disease Resistance
Plant Cells
Lycopersicon esculentum
Molecular Biology
Genes
Proteins

Keywords

  • Disease resistance response
  • Evans Blue
  • Hypersensitive response
  • Plant activator
  • Programmed cell death

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

A high-throughput chemical screening method for inhibitors and potentiators of hypersensitive cell death using suspension cell culture of Arabidopsis thaliana. / Noutoshi, Yoshiteru; Shirasu, Ken.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 39-47 (Methods in Molecular Biology; Vol. 1795).

Research output: Chapter in Book/Report/Conference proceedingChapter

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