A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation

Masayuki Matsushita, Kazuhito Tomizawa, Akiyoshi Moriwaki, Sheng Tian Li, Hiroaki Terada, Hideki Matsui

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that protein transduction by 11 arginine (11R) is an efficient method of delivering proteins into the neurons of brain slices. Here, we demonstrate that PKA inhibitory peptide, fused with 11R and nuclear localization signal, delivers the peptide exclusively into the nuclear compartment of neurons in brain slices. This inhibitory peptide blocked both cAMP responsive element-binding protein phosphorylation and long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight transduction of proteins and peptides into specific neuronal subcellular compartments in brain slices as a powerful tool for studying neuronal plasticity.

Original languageEnglish
Pages (from-to)6000-6007
Number of pages8
JournalJournal of Neuroscience
Volume21
Issue number16
DOIs
Publication statusPublished - Aug 15 2001

Keywords

  • Brain slice
  • CREB
  • LTP
  • PKA
  • Poly arginine
  • Protein transduction

ASJC Scopus subject areas

  • Neuroscience(all)

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