A high-density rice genetic linkage map with 2275 markers using a single F2 population

Yoshiaki Harushima, Masahiro Yano, Ayahiko Shomura, Mikiko Sato, Tomotoshi Shimano, Yoshihide Kuboki, Toshio Yamamoto, Shao Yang Lin, Baltazar A. Antonio, Arnold Parco, Hiromi Kajiya, Ning Huang, Kimiko Yamamoto, Yoshiaki Nagamura, Non Kurata, Gurdev S. Khush, Takuji Sasaki

Research output: Contribution to journalArticle

667 Citations (Scopus)

Abstract

A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using >130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high- resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.

Original languageEnglish
Pages (from-to)479-494
Number of pages16
JournalGenetics
Volume148
Issue number1
Publication statusPublished - Jan 1 1998
Externally publishedYes

Fingerprint

Genetic Linkage
Genetic Recombination
Centromere
Expressed Sequence Tags
Genetic Markers
Population
Genes
Chromosomes
Bony Callus
Isoenzymes
Libraries
Clone Cells
Genome
Oryza

ASJC Scopus subject areas

  • Genetics

Cite this

Harushima, Y., Yano, M., Shomura, A., Sato, M., Shimano, T., Kuboki, Y., ... Sasaki, T. (1998). A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics, 148(1), 479-494.

A high-density rice genetic linkage map with 2275 markers using a single F2 population. / Harushima, Yoshiaki; Yano, Masahiro; Shomura, Ayahiko; Sato, Mikiko; Shimano, Tomotoshi; Kuboki, Yoshihide; Yamamoto, Toshio; Lin, Shao Yang; Antonio, Baltazar A.; Parco, Arnold; Kajiya, Hiromi; Huang, Ning; Yamamoto, Kimiko; Nagamura, Yoshiaki; Kurata, Non; Khush, Gurdev S.; Sasaki, Takuji.

In: Genetics, Vol. 148, No. 1, 01.01.1998, p. 479-494.

Research output: Contribution to journalArticle

Harushima, Y, Yano, M, Shomura, A, Sato, M, Shimano, T, Kuboki, Y, Yamamoto, T, Lin, SY, Antonio, BA, Parco, A, Kajiya, H, Huang, N, Yamamoto, K, Nagamura, Y, Kurata, N, Khush, GS & Sasaki, T 1998, 'A high-density rice genetic linkage map with 2275 markers using a single F2 population', Genetics, vol. 148, no. 1, pp. 479-494.
Harushima Y, Yano M, Shomura A, Sato M, Shimano T, Kuboki Y et al. A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics. 1998 Jan 1;148(1):479-494.
Harushima, Yoshiaki ; Yano, Masahiro ; Shomura, Ayahiko ; Sato, Mikiko ; Shimano, Tomotoshi ; Kuboki, Yoshihide ; Yamamoto, Toshio ; Lin, Shao Yang ; Antonio, Baltazar A. ; Parco, Arnold ; Kajiya, Hiromi ; Huang, Ning ; Yamamoto, Kimiko ; Nagamura, Yoshiaki ; Kurata, Non ; Khush, Gurdev S. ; Sasaki, Takuji. / A high-density rice genetic linkage map with 2275 markers using a single F2 population. In: Genetics. 1998 ; Vol. 148, No. 1. pp. 479-494.
@article{a3022fc8355c4ab68bca7a6d89ea2582,
title = "A high-density rice genetic linkage map with 2275 markers using a single F2 population",
abstract = "A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using >130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high- resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.",
author = "Yoshiaki Harushima and Masahiro Yano and Ayahiko Shomura and Mikiko Sato and Tomotoshi Shimano and Yoshihide Kuboki and Toshio Yamamoto and Lin, {Shao Yang} and Antonio, {Baltazar A.} and Arnold Parco and Hiromi Kajiya and Ning Huang and Kimiko Yamamoto and Yoshiaki Nagamura and Non Kurata and Khush, {Gurdev S.} and Takuji Sasaki",
year = "1998",
month = "1",
day = "1",
language = "English",
volume = "148",
pages = "479--494",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "1",

}

TY - JOUR

T1 - A high-density rice genetic linkage map with 2275 markers using a single F2 population

AU - Harushima, Yoshiaki

AU - Yano, Masahiro

AU - Shomura, Ayahiko

AU - Sato, Mikiko

AU - Shimano, Tomotoshi

AU - Kuboki, Yoshihide

AU - Yamamoto, Toshio

AU - Lin, Shao Yang

AU - Antonio, Baltazar A.

AU - Parco, Arnold

AU - Kajiya, Hiromi

AU - Huang, Ning

AU - Yamamoto, Kimiko

AU - Nagamura, Yoshiaki

AU - Kurata, Non

AU - Khush, Gurdev S.

AU - Sasaki, Takuji

PY - 1998/1/1

Y1 - 1998/1/1

N2 - A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using >130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high- resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.

AB - A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using >130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high- resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.

UR - http://www.scopus.com/inward/record.url?scp=0031908205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031908205&partnerID=8YFLogxK

M3 - Article

VL - 148

SP - 479

EP - 494

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 1

ER -