A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators

Yunshan Li, Keinosuke Okamoto, Eizo Takahashi, Shin-ichi Miyoshi, Sumio Shinoda, Takao Tsuji, Yoshio Fujii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The hemolysin of Vibrio mimicus (VMH) is a pore-forming toxin with both enterotoxic and hemolytic activity. The hemolysis by VMH is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of VMH has remained unclear. In order to clarify the mechanism, we incubated T84 cells (a human colon carcinoma cell line) with VMH and found that the levels of ATP and cyclic AMP of culture medium increased after exposure of the cells to VMH. Subsequently, we found that the fluid accumulating activity off VMH in a mouse internal loop assay was reduced by administration of glibenclamide, an inhibitor of cyclic AMP-dependent chloride channels, into the intestinal loop. These results suggest that the stimulation of cells to produce nucleotides by VMH is linked to the enterotoxic activity of the toxin.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalMicrobiology and Immunology
Volume49
Issue number1
Publication statusPublished - 2005

Fingerprint

Cyclic AMP
Adenosine Triphosphate
Chloride Channels
Glyburide
Erythrocyte Membrane
Hemolysis
Culture Media
Colon
Nucleotides
Carcinoma
Cell Line
Vibrio hemolysin

Keywords

  • Cyclic AMP
  • Hemolysin
  • Pore-forming toxin
  • Vibrio mimicus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators. / Li, Yunshan; Okamoto, Keinosuke; Takahashi, Eizo; Miyoshi, Shin-ichi; Shinoda, Sumio; Tsuji, Takao; Fujii, Yoshio.

In: Microbiology and Immunology, Vol. 49, No. 1, 2005, p. 73-78.

Research output: Contribution to journalArticle

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AU - Shinoda, Sumio

AU - Tsuji, Takao

AU - Fujii, Yoshio

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