A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators

Yunshan Li, Keinosuke Okamoto, Eizo Takahashi, Shin Ichi Miyoshi, Sumio Shinoda, Takao Tsuji, Yoshio Fujii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The hemolysin of Vibrio mimicus (VMH) is a pore-forming toxin with both enterotoxic and hemolytic activity. The hemolysis by VMH is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of VMH has remained unclear. In order to clarify the mechanism, we incubated T84 cells (a human colon carcinoma cell line) with VMH and found that the levels of ATP and cyclic AMP of culture medium increased after exposure of the cells to VMH. Subsequently, we found that the fluid accumulating activity off VMH in a mouse internal loop assay was reduced by administration of glibenclamide, an inhibitor of cyclic AMP-dependent chloride channels, into the intestinal loop. These results suggest that the stimulation of cells to produce nucleotides by VMH is linked to the enterotoxic activity of the toxin.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 2005

Keywords

  • Cyclic AMP
  • Hemolysin
  • Pore-forming toxin
  • Vibrio mimicus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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