A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain

Xin Yuan Huang, Fenglin Deng, Naoki Yamaji, Shannon R M Pinson, Miho Fujii-Kashino, John Danku, Alex Douglas, Mary Lou Guerinot, David E. Salt, Jian Feng Ma

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.

Original languageEnglish
Article number12138
JournalNature Communications
Volume7
DOIs
Publication statusPublished - Jul 8 2016

Fingerprint

rice
heavy metals
Heavy Metals
Adenosine Triphosphatases
Copper
copper
nutrients
Nutrients
Food
Poisons
Quantitative Trait Loci
Amino Acid Substitution
loci
Vacuoles
genes
Minerals
amino acids
soils
Substitution reactions
Soil

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. / Huang, Xin Yuan; Deng, Fenglin; Yamaji, Naoki; Pinson, Shannon R M; Fujii-Kashino, Miho; Danku, John; Douglas, Alex; Guerinot, Mary Lou; Salt, David E.; Ma, Jian Feng.

In: Nature Communications, Vol. 7, 12138, 08.07.2016.

Research output: Contribution to journalArticle

Huang, XY, Deng, F, Yamaji, N, Pinson, SRM, Fujii-Kashino, M, Danku, J, Douglas, A, Guerinot, ML, Salt, DE & Ma, JF 2016, 'A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain', Nature Communications, vol. 7, 12138. https://doi.org/10.1038/ncomms12138
Huang, Xin Yuan ; Deng, Fenglin ; Yamaji, Naoki ; Pinson, Shannon R M ; Fujii-Kashino, Miho ; Danku, John ; Douglas, Alex ; Guerinot, Mary Lou ; Salt, David E. ; Ma, Jian Feng. / A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. In: Nature Communications. 2016 ; Vol. 7.
@article{9d74f9cb85cf4fc79283d5ade4041293,
title = "A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain",
abstract = "Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.",
author = "Huang, {Xin Yuan} and Fenglin Deng and Naoki Yamaji and Pinson, {Shannon R M} and Miho Fujii-Kashino and John Danku and Alex Douglas and Guerinot, {Mary Lou} and Salt, {David E.} and Ma, {Jian Feng}",
year = "2016",
month = "7",
day = "8",
doi = "10.1038/ncomms12138",
language = "English",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain

AU - Huang, Xin Yuan

AU - Deng, Fenglin

AU - Yamaji, Naoki

AU - Pinson, Shannon R M

AU - Fujii-Kashino, Miho

AU - Danku, John

AU - Douglas, Alex

AU - Guerinot, Mary Lou

AU - Salt, David E.

AU - Ma, Jian Feng

PY - 2016/7/8

Y1 - 2016/7/8

N2 - Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.

AB - Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs.

UR - http://www.scopus.com/inward/record.url?scp=84978160998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978160998&partnerID=8YFLogxK

U2 - 10.1038/ncomms12138

DO - 10.1038/ncomms12138

M3 - Article

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12138

ER -