A half-type AvABCG1 transporter derived from Andropogon virginicus L. confers aluminum tolerance

Bunichi Ezaki, Kenkou Takahashi, Kaori Utsumi, Aiko Higashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aluminum (Al) induced genes were isolated from a wild plant, Andropogon virginicus L. by a method involving arbitrarily primed PCR-based RNA finger printing. One of the clones, AP18-2-1, was induced in both roots and shoots by Al and diamide treatments, and slightly by Cu and Zn treatments. Full-length cDNA (2287. bp) was obtained by RACE (rapid amplification of cDNA ends) PCR and DNA sequencing indicated that it encoded a half-type ABC transporter that was highly homologous to other ABCG family genes. The Al-induced transporter gene derived from A. virginicus L. was therefore designated AvABCG1. The AvABCG1 gene conferred tolerance to Al, Cu and diamide in Arabidopsis thaliana transformants. A fusion protein (AvABCG1::GFP) was localized in the cell membrane region. Immunohistological analysis of the native protein in A. virginicus L. indicated that it localized to both exodermis and endodermis in roots, and to the vascular bundle sheath and its extensions in both upper and lower epidermal cells in leaves. Fluorescent measurement showed that absorbed Al was detected in almost the same areas as the AvABCG1 protein in both root and leaf. We conclude that the AvABCG1 transporter may confer Al tolerance by accumulating absorbed Al ions in specific regions of the plant.

Original languageEnglish
Pages (from-to)21-31
Number of pages11
JournalEnvironmental and Experimental Botany
Volume118
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Andropogon virginicus
aluminum
transporters
tolerance
gene
protein
genes
endodermis
ABC transporters
rapid amplification of cDNA ends
proteins
wild plants
vascular bundles
cell membranes
RNA
leaves
clone
amplification
sequence analysis
Arabidopsis thaliana

Keywords

  • A half-type ABCG transporter gene
  • Accumulation of metal
  • Al-induced gene
  • Al-tolerant gene
  • Aluminum (Al) stress
  • Pleiotropic drug resistance

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Agronomy and Crop Science

Cite this

A half-type AvABCG1 transporter derived from Andropogon virginicus L. confers aluminum tolerance. / Ezaki, Bunichi; Takahashi, Kenkou; Utsumi, Kaori; Higashi, Aiko.

In: Environmental and Experimental Botany, Vol. 118, 01.10.2015, p. 21-31.

Research output: Contribution to journalArticle

Ezaki, Bunichi ; Takahashi, Kenkou ; Utsumi, Kaori ; Higashi, Aiko. / A half-type AvABCG1 transporter derived from Andropogon virginicus L. confers aluminum tolerance. In: Environmental and Experimental Botany. 2015 ; Vol. 118. pp. 21-31.
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