A gold nano-electrode for single ion channel recordings

Daichi Okuno, Minako Hirano, Hiroaki Yokota, Junya Ichinose, Takamitsu Kira, Taiki Hijiya, Chihiro Uozumi, Masahiro Yamakami, Toru Ide

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The artificial bilayer single channel recording technique is commonly used to observe the detailed physiological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we developed a novel artificial bilayer single channel recording technique in which solubilized ion channel proteins immobilized on a gold nano-electrode are directly incorporated into a lipid bilayer at the same time as the bilayer is formed at the tip of it on coming in contact with an aqueous-oil interface. Using this technique, we measured the single channel currents of several types of channels including KcsA, MthK, hBK and P2X4. This technique requires only one action to simultaneously form the bilayers and reconstitute the channels into the membranes. This simplicity greatly increases the measurement efficiency and allows the technique to potentially be combined with high-throughput screening devices.

Original languageEnglish
Pages (from-to)4036-4040
Number of pages5
JournalNanoscale
Volume10
Issue number8
DOIs
Publication statusPublished - Feb 28 2018

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Ion Channels
Gold
Screening
Immobilized Proteins
Proteins
Electrodes
Lipid bilayers
Ions
Membrane Lipids
Oils
Throughput
Membranes
Chemical analysis

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Okuno, D., Hirano, M., Yokota, H., Ichinose, J., Kira, T., Hijiya, T., ... Ide, T. (2018). A gold nano-electrode for single ion channel recordings. Nanoscale, 10(8), 4036-4040. https://doi.org/10.1039/c7nr08098k

A gold nano-electrode for single ion channel recordings. / Okuno, Daichi; Hirano, Minako; Yokota, Hiroaki; Ichinose, Junya; Kira, Takamitsu; Hijiya, Taiki; Uozumi, Chihiro; Yamakami, Masahiro; Ide, Toru.

In: Nanoscale, Vol. 10, No. 8, 28.02.2018, p. 4036-4040.

Research output: Contribution to journalArticle

Okuno, D, Hirano, M, Yokota, H, Ichinose, J, Kira, T, Hijiya, T, Uozumi, C, Yamakami, M & Ide, T 2018, 'A gold nano-electrode for single ion channel recordings', Nanoscale, vol. 10, no. 8, pp. 4036-4040. https://doi.org/10.1039/c7nr08098k
Okuno D, Hirano M, Yokota H, Ichinose J, Kira T, Hijiya T et al. A gold nano-electrode for single ion channel recordings. Nanoscale. 2018 Feb 28;10(8):4036-4040. https://doi.org/10.1039/c7nr08098k
Okuno, Daichi ; Hirano, Minako ; Yokota, Hiroaki ; Ichinose, Junya ; Kira, Takamitsu ; Hijiya, Taiki ; Uozumi, Chihiro ; Yamakami, Masahiro ; Ide, Toru. / A gold nano-electrode for single ion channel recordings. In: Nanoscale. 2018 ; Vol. 10, No. 8. pp. 4036-4040.
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