A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin

Jiafu Tan, Lili Tu, Fenglin Deng, Haiyan Hu, Yichun Nie, Xianlong Zhang

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.

Original languageEnglish
Pages (from-to)86-95
Number of pages10
JournalPlant Physiology
Volume162
Issue number1
DOIs
Publication statusPublished - May 2013
Externally publishedYes

Fingerprint

Cotton Fiber
fiber cells
naringenin
lint cotton
Flavonoids
flavonoids
Gossypium
metabolism
Gossypium hirsutum
cotton
ovule culture
Ovule
Gossypium barbadense
Plant Development
Plant Cells
RNA Interference
cytoskeleton
Cytoskeleton
RNA interference
cell differentiation

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin. / Tan, Jiafu; Tu, Lili; Deng, Fenglin; Hu, Haiyan; Nie, Yichun; Zhang, Xianlong.

In: Plant Physiology, Vol. 162, No. 1, 05.2013, p. 86-95.

Research output: Contribution to journalArticle

Tan, Jiafu ; Tu, Lili ; Deng, Fenglin ; Hu, Haiyan ; Nie, Yichun ; Zhang, Xianlong. / A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin. In: Plant Physiology. 2013 ; Vol. 162, No. 1. pp. 86-95.
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