Abstract
The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.
| Original language | English |
|---|---|
| Pages (from-to) | 86-95 |
| Number of pages | 10 |
| Journal | Plant Physiology |
| Volume | 162 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Plant Science
- Genetics
- Physiology
Cite this
A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin. / Tan, Jiafu; Tu, Lili; Deng, Fenglin; Hu, Haiyan; Nie, Yichun; Zhang, Xianlong.
In: Plant Physiology, Vol. 162, No. 1, 05.2013, p. 86-95.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin
AU - Tan, Jiafu
AU - Tu, Lili
AU - Deng, Fenglin
AU - Hu, Haiyan
AU - Nie, Yichun
AU - Zhang, Xianlong
PY - 2013/5
Y1 - 2013/5
N2 - The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.
AB - The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.
UR - http://www.scopus.com/inward/record.url?scp=84877030946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877030946&partnerID=8YFLogxK
U2 - 10.1104/pp.112.212142
DO - 10.1104/pp.112.212142
M3 - Article
C2 - 23535943
AN - SCOPUS:84877030946
VL - 162
SP - 86
EP - 95
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 1
ER -