A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana

Kazuo Shinozaki, Tsuyoshi Mizoguchi, Kenji Irie, Takashi Hirayama, Nobuaki Hayashida, Kazuko Yamaguchi-Shinozaki, Kunihiro Matsumoto

Research output: Contribution to journalArticle

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Abstract

We describe here the cloning and characterization of a cDNA encoding a protein kinase that has high sequence homology to members of the mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK or MEKK) family; this cDNA is named cATMEKK1 (Arabidopsis thaliana MAP kinase or ERK kinase kinase 1). The catalytic domain of the putative ATMEKK1 protein shows ≈40% identity with the amino acid sequences of the catalytic domains of MAP-KKKs (such as Byr2 from Schizosaccharomyces pombe, Ste11 from Saccharomyces cerevisiae, Bck1 from S. cerevisiae, MEKK from mouse, and NPK1 from tobacco). In yeast cells that overexpress ATMEKK1, the protein kinase replaces Ste11 in responding to mating pheromone. In this study, the expression of three protein kinases was examined by Northern blot analyses: ATMEKK1 (structurally related to MAPKKK), ATMPK3 (structurally related to MAPK), and ATPK19 (structurally related to ribosomal S6 kinase). The mRNA levels of these three protein kinases increased markedly and simultaneously in response to touch, cold, and salinity stress. These results suggest that MAP kinase cascades, which are thought to respond to a variety of extracellular signals, are regulated not only at the posttranslational level but also at the transcriptional level in plants and that MAP kinase cascades in plants may function in transducing signals in the presence of environmental stress.

Original languageEnglish
Pages (from-to)765-769
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number2
Publication statusPublished - Jan 23 1996
Externally publishedYes

Fingerprint

Ribosomal Protein S6 Kinases
MAP Kinase Kinase Kinases
Touch
Mitogen-Activated Protein Kinases
Dehydration
Arabidopsis
Protein Kinases
MAP Kinase Kinase Kinase 1
MAP Kinase Signaling System
Genes
Saccharomyces cerevisiae
Catalytic Domain
Complementary DNA
Schizosaccharomyces
Pheromones
Salinity
Sequence Homology
Northern Blotting
Tobacco
Organism Cloning

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. / Shinozaki, Kazuo; Mizoguchi, Tsuyoshi; Irie, Kenji; Hirayama, Takashi; Hayashida, Nobuaki; Yamaguchi-Shinozaki, Kazuko; Matsumoto, Kunihiro.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 2, 23.01.1996, p. 765-769.

Research output: Contribution to journalArticle

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abstract = "We describe here the cloning and characterization of a cDNA encoding a protein kinase that has high sequence homology to members of the mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK or MEKK) family; this cDNA is named cATMEKK1 (Arabidopsis thaliana MAP kinase or ERK kinase kinase 1). The catalytic domain of the putative ATMEKK1 protein shows ≈40{\%} identity with the amino acid sequences of the catalytic domains of MAP-KKKs (such as Byr2 from Schizosaccharomyces pombe, Ste11 from Saccharomyces cerevisiae, Bck1 from S. cerevisiae, MEKK from mouse, and NPK1 from tobacco). In yeast cells that overexpress ATMEKK1, the protein kinase replaces Ste11 in responding to mating pheromone. In this study, the expression of three protein kinases was examined by Northern blot analyses: ATMEKK1 (structurally related to MAPKKK), ATMPK3 (structurally related to MAPK), and ATPK19 (structurally related to ribosomal S6 kinase). The mRNA levels of these three protein kinases increased markedly and simultaneously in response to touch, cold, and salinity stress. These results suggest that MAP kinase cascades, which are thought to respond to a variety of extracellular signals, are regulated not only at the posttranslational level but also at the transcriptional level in plants and that MAP kinase cascades in plants may function in transducing signals in the presence of environmental stress.",
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