A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley

Chao Li, Guoxiong Chen, Kohei Mishina, Naoki Yamaji, Jian Feng Ma, Fumiko Yukuhiro, Akemi Tagiri, Cheng Liu, Mohammad Pourkheirandish, Nadia Anwar, Masaru Ohta, Pengshan Zhao, Udda Lundqvist, Xinrong Li, Takao Komatsuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The hydrophobic cuticle covers the surface of the most aerial organs of land plants. The barley mutant eceriferum-zv (cer-zv), which is hypersensitive to drought, is unable to accumulate a sufficient quantity of cutin in its leaf cuticle. The mutated locus has been mapped to a 0.02 cM segment in the pericentromeric region of chromosome 4H. As a map-based cloning approach to isolate the gene was therefore considered unlikely to be feasible, a comparison was instead made between the transcriptomes of the mutant and the wild type. In conjunction with extant genomic information, on the basis of predicted functionality, only two genes were considered likely to encode a product associated with cutin formation. When eight independent cer-zv mutant alleles were resequenced with respect to the two candidate genes, it was confirmed that the gene underlying the mutation in each allele encodes a Gly-Asp-Ser-Leu (GDSL)-motif esterase/acyltransferase/lipase. The gene was transcribed in the epidermis, and its product was exclusively deposited in cell wall at the boundary of the cuticle in the leaf elongation zone, coinciding with the major site of cutin deposition. CER-ZV is speculated to function in the deposition of cutin polymer. Its homologs were found in green algae, moss, and euphyllophytes, indicating that it is highly conserved in plant kingdom.

Original languageEnglish
Article numbere00025
JournalPlant Direct
Volume1
Issue number5
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Acyltransferases
acyltransferases
Esterases
Hordeum
water retention
cutin
Lipase
barley
esterases
Genes
cuticle
Water
gene
leaves
genes
water
mutants
allele
Alleles
Embryophyta

Keywords

  • abiotic stress
  • cell walls
  • cuticle/waxes
  • drought/water stress

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

Cite this

A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley. / Li, Chao; Chen, Guoxiong; Mishina, Kohei; Yamaji, Naoki; Ma, Jian Feng; Yukuhiro, Fumiko; Tagiri, Akemi; Liu, Cheng; Pourkheirandish, Mohammad; Anwar, Nadia; Ohta, Masaru; Zhao, Pengshan; Lundqvist, Udda; Li, Xinrong; Komatsuda, Takao.

In: Plant Direct, Vol. 1, No. 5, e00025, 01.01.2017.

Research output: Contribution to journalArticle

Li, C, Chen, G, Mishina, K, Yamaji, N, Ma, JF, Yukuhiro, F, Tagiri, A, Liu, C, Pourkheirandish, M, Anwar, N, Ohta, M, Zhao, P, Lundqvist, U, Li, X & Komatsuda, T 2017, 'A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley', Plant Direct, vol. 1, no. 5, e00025. https://doi.org/10.1002/pld3.25
Li, Chao ; Chen, Guoxiong ; Mishina, Kohei ; Yamaji, Naoki ; Ma, Jian Feng ; Yukuhiro, Fumiko ; Tagiri, Akemi ; Liu, Cheng ; Pourkheirandish, Mohammad ; Anwar, Nadia ; Ohta, Masaru ; Zhao, Pengshan ; Lundqvist, Udda ; Li, Xinrong ; Komatsuda, Takao. / A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley. In: Plant Direct. 2017 ; Vol. 1, No. 5.
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AU - Ma, Jian Feng

AU - Yukuhiro, Fumiko

AU - Tagiri, Akemi

AU - Liu, Cheng

AU - Pourkheirandish, Mohammad

AU - Anwar, Nadia

AU - Ohta, Masaru

AU - Zhao, Pengshan

AU - Lundqvist, Udda

AU - Li, Xinrong

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