A fungal Argonaute interferes with RNA interference

Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein- sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNAand retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced inΔmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNAmediated gene regulation pathways through a competition for sRNA.

    Original languageEnglish
    Pages (from-to)2495-2508
    Number of pages14
    JournalNucleic acids research
    Volume46
    Issue number5
    DOIs
    Publication statusPublished - Mar 16 2018

    ASJC Scopus subject areas

    • Genetics

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