A fungal Argonaute interferes with RNA interference

Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein- sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNAand retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced inΔmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNAmediated gene regulation pathways through a competition for sRNA.

Original languageEnglish
Pages (from-to)2495-2508
Number of pages14
JournalNucleic Acids Research
Volume46
Issue number5
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

RNA Interference
RNA
Gene Silencing
Magnaporthe
High-Throughput Nucleotide Sequencing
Retroelements
Gene Knockout Techniques
Proteins
Site-Directed Mutagenesis
Small Interfering RNA
Genes
Fungi
Population
Oryza

ASJC Scopus subject areas

  • Genetics

Cite this

Nguyen, Q., Iritani, A., Ohkita, S., Vu, B. V., Yokoya, K., Matsubara, A., ... Nakayashiki, H. (2018). A fungal Argonaute interferes with RNA interference. Nucleic Acids Research, 46(5), 2495-2508. https://doi.org/10.1093/nar/gkx1301

A fungal Argonaute interferes with RNA interference. / Nguyen, Quyet; Iritani, Akihide; Ohkita, Shuhei; Vu, Ba V.; Yokoya, Kana; Matsubara, Ai; Ikeda, Ken Ichi; Suzuki, Nobuhiro; Nakayashiki, Hitoshi.

In: Nucleic Acids Research, Vol. 46, No. 5, 01.01.2018, p. 2495-2508.

Research output: Contribution to journalArticle

Nguyen, Q, Iritani, A, Ohkita, S, Vu, BV, Yokoya, K, Matsubara, A, Ikeda, KI, Suzuki, N & Nakayashiki, H 2018, 'A fungal Argonaute interferes with RNA interference', Nucleic Acids Research, vol. 46, no. 5, pp. 2495-2508. https://doi.org/10.1093/nar/gkx1301
Nguyen Q, Iritani A, Ohkita S, Vu BV, Yokoya K, Matsubara A et al. A fungal Argonaute interferes with RNA interference. Nucleic Acids Research. 2018 Jan 1;46(5):2495-2508. https://doi.org/10.1093/nar/gkx1301
Nguyen, Quyet ; Iritani, Akihide ; Ohkita, Shuhei ; Vu, Ba V. ; Yokoya, Kana ; Matsubara, Ai ; Ikeda, Ken Ichi ; Suzuki, Nobuhiro ; Nakayashiki, Hitoshi. / A fungal Argonaute interferes with RNA interference. In: Nucleic Acids Research. 2018 ; Vol. 46, No. 5. pp. 2495-2508.
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