A fully automated pH measurement system for 96-well microplates using a semiconductor-based pH sensor

Akira Yamada, Satoshi Mohri, Michihiro Nakamura, Keiji Naruse

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The pH values of solutions have been commonly measured by glass electrodes, devices which respond on a time scale of minutes and require complicated operations such as calibration at every measurement. The development of a fast, automated pH measurement system for analytes in small volumes would be quite useful in many fields. Yet the practical application of such a device has been hampered by the need for rapid measurement and precise calibration. Our group has constructed a fully automated, self-calibrating pH measurement system capable of consecutively measuring 96 samples. The system uses a flow-through-type differential pH sensor probe based on a tiny, quickly responding pH-sensitive field-effect transistor (pH-FET) technology. The system was confirmed to measure the pH values of 26 μl solutions over a 135 s cycle (60 s for wash, 20 s for movement, 3 s for suction, and 52 s for measurement). The measurement error of multiple sample measurements, including measurements of solutions with low buffering capacity, was -0.0116 ± 0.0481 pH (average error ± S.D.). The high accuracy and high reproducibility of the results suggest that this automatic pH measurement system based on pH-FET can be applied for versatile uses.

Original languageEnglish
Pages (from-to)464-469
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume143
Issue number2
DOIs
Publication statusPublished - Jan 7 2010

Fingerprint

pH sensors
Semiconductor materials
sensors
Field effect transistors
Calibration
field effect transistors
glass electrodes
Measurement errors
suction
calibrating
Glass
Electrodes

Keywords

  • 96-Well microplate
  • Automation
  • Differential pH sensor probe
  • pH
  • pH-FET

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

A fully automated pH measurement system for 96-well microplates using a semiconductor-based pH sensor. / Yamada, Akira; Mohri, Satoshi; Nakamura, Michihiro; Naruse, Keiji.

In: Sensors and Actuators, B: Chemical, Vol. 143, No. 2, 07.01.2010, p. 464-469.

Research output: Contribution to journalArticle

@article{24fadfcf9e3643d79b312ae93a3dd8c5,
title = "A fully automated pH measurement system for 96-well microplates using a semiconductor-based pH sensor",
abstract = "The pH values of solutions have been commonly measured by glass electrodes, devices which respond on a time scale of minutes and require complicated operations such as calibration at every measurement. The development of a fast, automated pH measurement system for analytes in small volumes would be quite useful in many fields. Yet the practical application of such a device has been hampered by the need for rapid measurement and precise calibration. Our group has constructed a fully automated, self-calibrating pH measurement system capable of consecutively measuring 96 samples. The system uses a flow-through-type differential pH sensor probe based on a tiny, quickly responding pH-sensitive field-effect transistor (pH-FET) technology. The system was confirmed to measure the pH values of 26 μl solutions over a 135 s cycle (60 s for wash, 20 s for movement, 3 s for suction, and 52 s for measurement). The measurement error of multiple sample measurements, including measurements of solutions with low buffering capacity, was -0.0116 ± 0.0481 pH (average error ± S.D.). The high accuracy and high reproducibility of the results suggest that this automatic pH measurement system based on pH-FET can be applied for versatile uses.",
keywords = "96-Well microplate, Automation, Differential pH sensor probe, pH, pH-FET",
author = "Akira Yamada and Satoshi Mohri and Michihiro Nakamura and Keiji Naruse",
year = "2010",
month = "1",
day = "7",
doi = "10.1016/j.snb.2009.09.045",
language = "English",
volume = "143",
pages = "464--469",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - A fully automated pH measurement system for 96-well microplates using a semiconductor-based pH sensor

AU - Yamada, Akira

AU - Mohri, Satoshi

AU - Nakamura, Michihiro

AU - Naruse, Keiji

PY - 2010/1/7

Y1 - 2010/1/7

N2 - The pH values of solutions have been commonly measured by glass electrodes, devices which respond on a time scale of minutes and require complicated operations such as calibration at every measurement. The development of a fast, automated pH measurement system for analytes in small volumes would be quite useful in many fields. Yet the practical application of such a device has been hampered by the need for rapid measurement and precise calibration. Our group has constructed a fully automated, self-calibrating pH measurement system capable of consecutively measuring 96 samples. The system uses a flow-through-type differential pH sensor probe based on a tiny, quickly responding pH-sensitive field-effect transistor (pH-FET) technology. The system was confirmed to measure the pH values of 26 μl solutions over a 135 s cycle (60 s for wash, 20 s for movement, 3 s for suction, and 52 s for measurement). The measurement error of multiple sample measurements, including measurements of solutions with low buffering capacity, was -0.0116 ± 0.0481 pH (average error ± S.D.). The high accuracy and high reproducibility of the results suggest that this automatic pH measurement system based on pH-FET can be applied for versatile uses.

AB - The pH values of solutions have been commonly measured by glass electrodes, devices which respond on a time scale of minutes and require complicated operations such as calibration at every measurement. The development of a fast, automated pH measurement system for analytes in small volumes would be quite useful in many fields. Yet the practical application of such a device has been hampered by the need for rapid measurement and precise calibration. Our group has constructed a fully automated, self-calibrating pH measurement system capable of consecutively measuring 96 samples. The system uses a flow-through-type differential pH sensor probe based on a tiny, quickly responding pH-sensitive field-effect transistor (pH-FET) technology. The system was confirmed to measure the pH values of 26 μl solutions over a 135 s cycle (60 s for wash, 20 s for movement, 3 s for suction, and 52 s for measurement). The measurement error of multiple sample measurements, including measurements of solutions with low buffering capacity, was -0.0116 ± 0.0481 pH (average error ± S.D.). The high accuracy and high reproducibility of the results suggest that this automatic pH measurement system based on pH-FET can be applied for versatile uses.

KW - 96-Well microplate

KW - Automation

KW - Differential pH sensor probe

KW - pH

KW - pH-FET

UR - http://www.scopus.com/inward/record.url?scp=73149103682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73149103682&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2009.09.045

DO - 10.1016/j.snb.2009.09.045

M3 - Article

VL - 143

SP - 464

EP - 469

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 2

ER -