A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and function in 3T3-L1 cells

Daigo Abe, Takeshi Saito, Yasutaka Kubo, Yoshimasa Nakamura, Keizo Sekiya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Adipocyte dysfunction is strongly associated with the development of insulin resistance and diabetes, and regulation of adipogenesis is important in prevention of diabetes. We prepared a 100% methanol fraction of methanolic extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit methanol fraction). When applied to 3T3-L1 preadipocyte cells, KMF promoted adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) activity, and increased triglyceride (TG) content. KMF markedly increased mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ)-the master adipogenic transcription factor-and its target genes. Moreover, KMF increased mRNA expression and protein secretion of adiponectin, whereas mRNA expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased the production of reactive oxygen species (ROS) and nuclear factor-kappaB (NFκB) activation. Glucose uptake was stimulated by KMF in differentiated 3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert beneficial effects against diabetes via its ability to regulate adipocyte differentiation and function.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalBioFactors
Volume36
Issue number1
DOIs
Publication statusPublished - Apr 26 2010

Keywords

  • Adipocyte
  • Adipocytokine
  • Diabetes
  • Kiwi fruit

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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