A flagellar gene fliZ regulates the expression of invasion genes and virulence phenotype in Salmonella enterica serovar Typhimurium

Sunao Iyoda, Tomokazu Kamidoi, Kenji Hirose, Kazuhiro Kutsukake, Haruo Watanabe

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We previously reported that the fliZ gene encodes a positive regulatory factor for the class 2 flagellar operons in Salmonella enterica serovar Typhimurium. In this study, we found that the fliZ mutation reduced not only the amounts of excreted flagellar proteins, but also those of several secreted invasion proteins encoded by the genes within Salmonella pathogenicity island 1. Using the lacZ gene fused to a subset of virulence-associated genes, we show that this downregulation was caused by a decreased transcription of the hilA gene, which encodes a positive regulator for the invasion genes. We further show that the fliZ mutation reduced invasion ability of S. enterica serovar Typhimurium to HEp-2 cells. Consistent with these results, orally challenged cells of the fliZ mutant show an attenuated virulence phenotype in a mouse typhoid model. These results indicate that the fliZ gene product positively regulates the invasion genes and is necessary for expression of full virulence.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalMicrobial Pathogenesis
Volume30
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Salmonella enterica
Virulence
Phenotype
Gene Expression
Genes
Genomic Islands
Mutation
Lac Operon
Typhoid Fever
Regulator Genes
Operon
Salmonella
Proteins
Down-Regulation
Serogroup

Keywords

  • Flagellar regulon
  • FliZ
  • Salmonella enterica serovar Typhimurium
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

A flagellar gene fliZ regulates the expression of invasion genes and virulence phenotype in Salmonella enterica serovar Typhimurium. / Iyoda, Sunao; Kamidoi, Tomokazu; Hirose, Kenji; Kutsukake, Kazuhiro; Watanabe, Haruo.

In: Microbial Pathogenesis, Vol. 30, No. 2, 2001, p. 81-90.

Research output: Contribution to journalArticle

Iyoda, Sunao ; Kamidoi, Tomokazu ; Hirose, Kenji ; Kutsukake, Kazuhiro ; Watanabe, Haruo. / A flagellar gene fliZ regulates the expression of invasion genes and virulence phenotype in Salmonella enterica serovar Typhimurium. In: Microbial Pathogenesis. 2001 ; Vol. 30, No. 2. pp. 81-90.
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