A factor in conditioned medium of rabbit costal chondrocytes inhibits the proliferation of cultured endothelial cells and angiogenesis induced by B16 melanoma: Its relation with cartilage-derived anti-tumor factor (CATF)

Masaharu Takigawa, E. Shirai, M. Enomoto, H. O. Pan, F. Suzuki, T. Shiio, Y. Yugari

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Serum-free medium conditioned by exposure to rabbit costal chondrocytes in culture inhibited the proliferation and DNA synthesis of bovine pulmonary endothelial cells in culture. The factor in conditioned medium did not inhibit DNA synthesis in B16 melanoma cells, L1210 cells or 3T3 fibroblasts in culture, but it inhibited angiogenesis in the chorioallantoic membrane of chick embryos induced by B16 melanoma and growth of the tumor transplanted onto the membrane. These findings strongly suggest that rabbit costal chondrocytes produce an anti-angiogenesis factor that is similar to cartilage-derived anti-tumor factor (CATF).

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalBiochemistry International
Volume14
Issue number2
Publication statusPublished - 1987
Externally publishedYes

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Experimental Melanomas
Endothelial cells
Cartilage
Conditioned Culture Medium
Chondrocytes
Cell culture
Tumors
Cultured Cells
Endothelial Cells
Rabbits
Membranes
Chorioallantoic Membrane
3T3 Cells
Angiogenesis Inducing Agents
DNA
Serum-Free Culture Media
Chick Embryo
Fibroblasts
Neoplasms
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

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AU - Shirai, E.

AU - Enomoto, M.

AU - Pan, H. O.

AU - Suzuki, F.

AU - Shiio, T.

AU - Yugari, Y.

PY - 1987

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