A dual role of FGF10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development

Hirotaka Tao, Miyuki Shimizu, Ryo Kusumoto, Katsuhiko Ono, Sumihare Noji, Hideyo Ohuchi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The development of the eyelid requires coordinated cellular processes of proliferation, cell shape changes, migration and cell death. Mutant mice deficient in the fibroblast growth factor 10 (Fgf10) gene exhibit open-eyelids at birth. To elucidate the roles of FGF10 during eyelid formation, we examined the expression pattern of Fgf10 during eyelid formation and the phenotype of Fgf10-null eyelids in detail. Fgf10 is expressed by mesenchymal cells just beneath the protruding epidermal cells of the nascent eyelid. However, Fgf10-null epithelial cells running though the eyelid groove do not exhibit typical cuboid shape or sufficient proliferation. Furthermore, peridermal clumps are not maintained on the eyelid leading edge, and epithelial extension does not occur. At the cellular level, the accumulation of actin fibers is not observed in the mutant epithelial leading edge. The expression of activin/inhibin βB (ActβB/Inhbb) and transforming growth factor α (Tgfa), previously reported to be crucial for eyelid development, is down-regulated in the mutant leading edge, while the onset of sonic hedgehog (Shh) expression is delayed on the mutant eyelid margin. Explant cultures of mouse eyelid primordia shows that the open-eyelid phenotype of the mutant is reduced by exogenous FGF10 protein, and that the expression of ActβB and Tgfa is ectopically induced in the thickened eyelid epithelium by the FGF10 protein. These results indicate a dual role of FGF10 in mouse eyelid development, for both proliferation and coordinated migration of eyelid epithelial cells by reorganization of the cytoskeleton, through the regulation of activin, TGFα and SHH signaling.

Original languageEnglish
Pages (from-to)3217-3230
Number of pages14
JournalDevelopment
Volume132
Issue number14
DOIs
Publication statusPublished - Jul 2005
Externally publishedYes

Fingerprint

Eyelids
Fibroblast Growth Factor 10
Transforming Growth Factors
Epithelial Cells
Phenotype
Activins
Null Lymphocytes
Hedgehogs
Cell Shape
Cytoskeleton
Running
Actins
Proteins
Cell Death

Keywords

  • Activin βB
  • Cell migration
  • Epidermis
  • Eyelid development
  • Fgf10
  • Leading edge
  • Mesenchyme
  • Mouse
  • Periderm
  • Shh
  • Tgfa

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

A dual role of FGF10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development. / Tao, Hirotaka; Shimizu, Miyuki; Kusumoto, Ryo; Ono, Katsuhiko; Noji, Sumihare; Ohuchi, Hideyo.

In: Development, Vol. 132, No. 14, 07.2005, p. 3217-3230.

Research output: Contribution to journalArticle

Tao, Hirotaka ; Shimizu, Miyuki ; Kusumoto, Ryo ; Ono, Katsuhiko ; Noji, Sumihare ; Ohuchi, Hideyo. / A dual role of FGF10 in proliferation and coordinated migration of epithelial leading edge cells during mouse eyelid development. In: Development. 2005 ; Vol. 132, No. 14. pp. 3217-3230.
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