A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos

Hidefumi Yoshioka, Hideyo Ohuchi, Yoshiyasu Ishimaru, Tetsuya Ishikawa, Tsutomu Nohno, Kaoru Saigo, Sumihare Noji

Research output: Contribution to journalArticle

Abstract

Members of the fibroblast growth factor (FGF) family play important roles in various developmental processes in vertebrates. Since two genes closely related to the vertebrate FGF receptor (FGFR) genes DFR1 and DFR2/breathless have already been reported in Drosophila, the existence of a Drosophila FGF has been predicted. In the present study, we examined whether DFR1 is functionally interchangeable with a vertebrate FGFR in the Xenopus system. First, we found that the expression of DFR1 promoted Ca2+ efflux in response to human basic (b)FGF in Xenopus oocytes, whereas the coexpression of a dominant negative form of DFR1 (ΔDFR1) with a chick FGFR1/cek1 inhibited promotion of Ca2+ efflux induced by the expression of cek1 in the oocyte. Second, the expression of ΔDFR1 was observed to induce a defect in the posterior structure of the Xenopus embryo at stage 30, as observed with a dominant negative form of cek1 (Δcek1). Third, we found that the expression of ΔDFR1 inhibited the expression of FGF-regulated genes such as Xbra, Xnot, and Xshh in Xenopus embryos at stage 11, while the coexpression of DFR1 with ΔDFR1 could rescue the inhibited expression of FGF-regulated genes. These results indicate that DFR1 acts as an FGFR in Xenopus embryos and that an FGF is likely to exist in Drosophila.

Original languageEnglish
Pages (from-to)617-624
Number of pages8
JournalDevelopment Growth and Differentiation
Volume38
Issue number6
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Fibroblast Growth Factor Receptors
Fibroblast Growth Factors
Receptor Protein-Tyrosine Kinases
Xenopus
Drosophila
Embryonic Structures
Vertebrates
Genes
Oocytes
Fibroblast Growth Factor 2

Keywords

  • Ca efflux
  • DFR1
  • Drosophila
  • fibroblast growth factor receptor
  • Xenopus laevis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy

Cite this

A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos. / Yoshioka, Hidefumi; Ohuchi, Hideyo; Ishimaru, Yoshiyasu; Ishikawa, Tetsuya; Nohno, Tsutomu; Saigo, Kaoru; Noji, Sumihare.

In: Development Growth and Differentiation, Vol. 38, No. 6, 1996, p. 617-624.

Research output: Contribution to journalArticle

Yoshioka, Hidefumi ; Ohuchi, Hideyo ; Ishimaru, Yoshiyasu ; Ishikawa, Tetsuya ; Nohno, Tsutomu ; Saigo, Kaoru ; Noji, Sumihare. / A Drosophila receptor-type tyrosine kinase (DFR1) acts as a fibroblast growth factor receptor in Xenopus embryos. In: Development Growth and Differentiation. 1996 ; Vol. 38, No. 6. pp. 617-624.
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