TY - JOUR
T1 - A distinct function of the retinoblastoma protein in the control of lipid composition identified by lipidomic profiling
AU - Muranaka, H.
AU - Hayashi, A.
AU - Minami, K.
AU - Kitajima, S.
AU - Kohno, S.
AU - Nishimoto, Y.
AU - Nagatani, N.
AU - Suzuki, M.
AU - Kulathunga, L. A.N.
AU - Sasaki, N.
AU - Okada, N.
AU - Matsuzaka, T.
AU - Shimano, H.
AU - Tada, H.
AU - Takahashi, C.
N1 - Funding Information:
We thank Drs K. Kishikawa and T Okazaki for introduction to lipidomics techniques, Drs M Asano, T Daikoku and E Kamimura for support in animal experiments, Drs K Ito, A Ishimura, S Tange, K Kajiwara and J Zhao for technical assistance and discussion, all colleagues in Takahashi’s laboratory especially Drs A Shamma, Y Kido, N Hayashi, A Yoshida, S Mohammed, Mrs F Li and Y Wan and in Cancer Research Institute for support. This work was supported by Founding Program for Next Generation World-Leading Researchers (NEXT), Grant-in-Aid for Scientific Research (JSPS KAKENHI), a research grant from Japan Agency for Medical Research and Development (AMED), Astellas Foundation for Research on Metabolic Disorders, Daiichi-Sankyo Foundation for Life Science, Hokkoku Foundation for Cancer Research, Naito Foundation, NOVARTIS Foundation for promotion of Science, Ono Medical Research Foundation and Takeda Science Foundation to C Takahashi, and the Sasakawa Scientific Research Grant and Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas Ministry of Education, Culture, Sports, Science and Technology to H Muranaka.
Publisher Copyright:
© 2017, The Author(s).
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Here, by combining lipidomics with transcriptome analysis, we demonstrate that Rb depletion in mouse embryonic fibroblastss induces significant alterations in their lipid composition. We discovered that Rb depletion induced increase in lysophosphatidylserine, diacylglycerol (DAG), fatty acid (FA), acylcarnitine, phosphatidylcholine (PC), arachidonoyl ethanolamine, and decrease in phosphatidylglycerol, monoacylglycerol, without change in total lipid per protein levels. Analysis of the acyl chain composition of DAG, PC and phosphatidylserine revealed increase of saturated and mono-unsaturated acyl chains with specific carbon chain length. Consistently, we observed that Rb depletion increased the levels of fatty acids with the corresponding carbon chain length and number of carbon–carbon double bondssuch as myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0) and all forms of FA 18:1. Microarray analysis revealed that Rb depletion induced significant upregulation of enzymes involved in elongation and desaturation of fatty acids. Among these, we found that elongation of long chain fatty acid family member 6 (Elovl6) and stearoyl-CoA desaturase 1 (Scd1) are the most robustly controlled by Rb possibly through E2F and sterol regulatory element-binding protein transcription factors. Depletion of Elovl6 or Scd1 significantly suppressed colony formation, sphere formation and xenograft tumor growth of Rb-deficient tumor cells. Suppression of self-renewal by the SCD1 inhibitor was rescued upon supplementation of the mono-unsaturated fatty acids generated by this enzyme. This study suggests a novel role for Rb in suppressing the malignant progression of tumors by controlling the lipid composition.
AB - Here, by combining lipidomics with transcriptome analysis, we demonstrate that Rb depletion in mouse embryonic fibroblastss induces significant alterations in their lipid composition. We discovered that Rb depletion induced increase in lysophosphatidylserine, diacylglycerol (DAG), fatty acid (FA), acylcarnitine, phosphatidylcholine (PC), arachidonoyl ethanolamine, and decrease in phosphatidylglycerol, monoacylglycerol, without change in total lipid per protein levels. Analysis of the acyl chain composition of DAG, PC and phosphatidylserine revealed increase of saturated and mono-unsaturated acyl chains with specific carbon chain length. Consistently, we observed that Rb depletion increased the levels of fatty acids with the corresponding carbon chain length and number of carbon–carbon double bondssuch as myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0) and all forms of FA 18:1. Microarray analysis revealed that Rb depletion induced significant upregulation of enzymes involved in elongation and desaturation of fatty acids. Among these, we found that elongation of long chain fatty acid family member 6 (Elovl6) and stearoyl-CoA desaturase 1 (Scd1) are the most robustly controlled by Rb possibly through E2F and sterol regulatory element-binding protein transcription factors. Depletion of Elovl6 or Scd1 significantly suppressed colony formation, sphere formation and xenograft tumor growth of Rb-deficient tumor cells. Suppression of self-renewal by the SCD1 inhibitor was rescued upon supplementation of the mono-unsaturated fatty acids generated by this enzyme. This study suggests a novel role for Rb in suppressing the malignant progression of tumors by controlling the lipid composition.
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U2 - 10.1038/oncsis.2017.51
DO - 10.1038/oncsis.2017.51
M3 - Article
AN - SCOPUS:85033237621
VL - 6
SP - e350
JO - Oncogenesis
JF - Oncogenesis
SN - 2157-9024
IS - 6
ER -