A dicarboxylate transporter, LjALMT4, mainly expressed in nodules of lotus japonicus

Kojiro Takanashi, Takayuki Sasaki, Tomohiro Kan, Yuka Saida, Akifumi Sugiyama, Yoko Yamamoto, Kazufumi Yazaki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Legume plants can establish symbiosis with soil bacteria called rhizobia to obtain nitrogen as a nutrient directly from atmospheric N2 via symbiotic nitrogen fixation. Legumes and rhizobia form nodules, symbiotic organs in which fixed-nitrogen and photosynthetic products are exchanged between rhizobia and plant cells. The photosynthetic products supplied to rhizobia are thought to be dicarboxylates but little is known about the movement of dicarboxylates in the nodules. In terms of dicarboxylate transporters, an aluminum-activated malate transporter (ALMT) family is a strong candidate responsible for the membrane transport of carboxylates in nodules. Among the seven ALMT genes in the Lotus japonicus genome, only one, LjALMT4, shows a high expression in the nodules. LjALMT4 showed transport activity in a Xenopus oocyte system, with LjALMT4 mediating the efflux of dicarboxylates including malate, succinate, and fumarate, but not tricarboxylates such as citrate. LjALMT4 also mediated the influx of several inorganic anions. Organ-specific gene expression analysis showed LjALMT4 mRNA mainly in the parenchyma cells of nodule vascular bundles. These results suggest that LjALMT4 may not be involved in the direct supply of dicarboxylates to rhizobia in infected cells but is responsible for supplying malate as well as several anions necessary for symbiotic nitrogen fixation, via nodule vasculatures.

Original languageEnglish
Pages (from-to)584-592
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume29
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

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Dicarboxylic Acid Transporters
Lotus corniculatus var. japonicus
Rhizobium
transporters
malates
Nitrogen Fixation
Aluminum
nitrogen fixation
Fabaceae
anions
Anions
aluminum
Nitrogen
legumes
Fumarates
Symbiosis
Plant Cells
soil bacteria
Xenopus
vascular bundles

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

A dicarboxylate transporter, LjALMT4, mainly expressed in nodules of lotus japonicus. / Takanashi, Kojiro; Sasaki, Takayuki; Kan, Tomohiro; Saida, Yuka; Sugiyama, Akifumi; Yamamoto, Yoko; Yazaki, Kazufumi.

In: Molecular Plant-Microbe Interactions, Vol. 29, No. 7, 01.07.2016, p. 584-592.

Research output: Contribution to journalArticle

Takanashi, Kojiro ; Sasaki, Takayuki ; Kan, Tomohiro ; Saida, Yuka ; Sugiyama, Akifumi ; Yamamoto, Yoko ; Yazaki, Kazufumi. / A dicarboxylate transporter, LjALMT4, mainly expressed in nodules of lotus japonicus. In: Molecular Plant-Microbe Interactions. 2016 ; Vol. 29, No. 7. pp. 584-592.
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