A cryptic Rab1-binding site in the p115 tethering protein

Matthew Beard, Ayano Satoh, James Shorter, Graham Warren

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Small GTPases and coiled-coil proteins of the golgin family help to tether COPI vesicles to Golgi membranes. At the cis-side of the Golgi, the Rab1 GTPase binds directly to each of three coiled-coil proteins: p115, GM130, and as now shown, Giantin. Rab1 binds to a coiled-coil region within the tail domain of p115 and this binding is inhibited by the C-terminal, acidic domain of p115. Furthermore, GM130 and Giantin bind to the acidic domain of p115 and stimulate p115 binding to Rab1, suggesting that p115 binding to Rab1 is regulated. Regulation of this interaction by proteins such as GM130 and Giantin may control the membrane recruitment of p115 by Rab1.

Original languageEnglish
Pages (from-to)25840-25848
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number27
DOIs
Publication statusPublished - Jul 8 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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