A coplanar PCB induces a selenium binding protein as a major cytosolic protein in rat liver

Yuji Ishii, Megumu Hatsumura, Takumi Ishida, Noritaka Ariyoshi, Kazuta Oguri

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We obtained evidence that a toxic coplanar polychlorinated biphenyl (PCB) induces a counterpart,of murine 56 kDa selenium binding protein in rat liver cytosol. A 54 kDa protein in the liver cytosol was significantly induced by 3,3',4,4',5-pentachlorobiphenyl and proved to be a major cytosolic protein in the rat liver. The protein exhibited pi of 6.8 on two-dimensional gel electrophoresis. The amino acid sequence of peptide fragments from the protein digested in situ, was highly similar to a 56 kDa selenium binding protein and similar to an acetaminophen binding protein in mice.

Original languageEnglish
Pages (from-to)509-515
Number of pages7
JournalChemosphere
Volume32
Issue number3
DOIs
Publication statusPublished - Feb 1996
Externally publishedYes

Fingerprint

Selenium-Binding Proteins
Polychlorinated Biphenyls
Selenium
Polychlorinated biphenyls
selenium
Liver
Rats
PCB
Proteins
3,4,5,3',4'-pentachlorobiphenyl
protein
Cytosol
Peptide Fragments
Poisons
Electrophoresis, Gel, Two-Dimensional
Acetaminophen
Electrophoresis
Peptides
Amino acids
Amino Acid Sequence

Keywords

  • 3,3',4,4,'5-pentachlorobiphenyl
  • Acetaminophen binding protein
  • Induction
  • PCB 126
  • Polychlorinated biphenyl (PCB)
  • Selenium
  • Selenium binding protein

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

A coplanar PCB induces a selenium binding protein as a major cytosolic protein in rat liver. / Ishii, Yuji; Hatsumura, Megumu; Ishida, Takumi; Ariyoshi, Noritaka; Oguri, Kazuta.

In: Chemosphere, Vol. 32, No. 3, 02.1996, p. 509-515.

Research output: Contribution to journalArticle

Ishii, Yuji ; Hatsumura, Megumu ; Ishida, Takumi ; Ariyoshi, Noritaka ; Oguri, Kazuta. / A coplanar PCB induces a selenium binding protein as a major cytosolic protein in rat liver. In: Chemosphere. 1996 ; Vol. 32, No. 3. pp. 509-515.
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