A Convenient Method for Determining the Effect of Abscisic Acid on the Stomatal Aperture of Rice (Oryza Sativa L.) by Feeding Solution to the Leaf Tip

Toru Kobata, Shinichi Hara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We established a convenient method for determining the effect of abscisic acid (ABA) solution on the stomatal aperture in wilting-sensitive plants such as rice (Oryza staiva L.). Water or ABA solution in a small vial was fed into a leaf tip under field conditions. Stomatal conductance was decreased by concentrations of ABA from 10−3 to 10−8 mol L−1 three hours after application. The amount of solution fed into the plant depended on the transpiration rate of the leaf. It was shown by the infiltration method that the stomata of an ABA-fed leaf closed first at the leaf edge and progressively toward the midrib. This heterogeneous stomatal closing may cause variance of stomatal conductance in the mid portion of the leaf in some cases. However if we can measure the stomatal conductance of portions of the leaf nearer to the tip, it may be possible to detect the stomatal response sooner and with less variation. We conclude that the assay method helps to define stomatal response to growth substances such as ABA of plants which suffer drought or any stress.

Original languageEnglish
Pages (from-to)638-642
Number of pages5
JournalJapanese Journal of Crop Science
Volume63
Issue number4
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

Abscisic Acid
abscisic acid
Oryza sativa
rice
stomatal conductance
leaves
Growth Substances
methodology
Droughts
Oryza
wilting
stomata
infiltration (hydrology)
transpiration
drought
Water
assays
water

Keywords

  • Abscisic acid
  • Oryza sativa L.
  • Stomatal conductance
  • Wilting

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Genetics

Cite this

A Convenient Method for Determining the Effect of Abscisic Acid on the Stomatal Aperture of Rice (Oryza Sativa L.) by Feeding Solution to the Leaf Tip. / Kobata, Toru; Hara, Shinichi.

In: Japanese Journal of Crop Science, Vol. 63, No. 4, 01.01.1994, p. 638-642.

Research output: Contribution to journalArticle

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