TY - JOUR
T1 - A Comparison of the Preservation of Mouse Adipose Tissue-Derived Mesenchymal Stem Cells Using the University of Wisconsin Solution and Hank's Balanced Salt Solution
AU - Nahar, Saifun
AU - Nakashima, Yoshiki
AU - Miyagi-Shiohira, Chika
AU - Kinjo, Takao
AU - Kobayashi, Naoya
AU - Saitoh, Issei
AU - Watanabe, Masami
AU - Noguchi, Hirofumi
AU - Fujita, Jiro
N1 - Funding Information:
We thank Naomi Kakazu (University of the Ryukyus) for clerical assistance and Saki Uema, Yuka Onishi, Maki Higa, Yuki Kawahira, and Saori Adaniya (University of the Ryukyus) for providing technical support. This work was supported by the Research Laboratory Center, Faculty of Medicine, and the Institute for Animal Experiments, Faculty of Medicine, University of the Ryukyus. This work was supported in part by the Japan Society for the Promotion of Science (JSPS; KAKENHI Grant number 16H07094), Japan Agency for Medical Research and Development, Naito Foundation, and Okinawa Science and Technology Promotion Center (OSTC).
Publisher Copyright:
© 2018 Saifun Nahar et al.
PY - 2018
Y1 - 2018
N2 - Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank's balanced salt solution (HBSS) is one of the main ADSC preservation solutions used clinically. However, this step is known to lead to decreased cell viability. The University of Wisconsin (UW) solution is recognized by transplant physicians as an excellent organ preservation solution. We aimed to investigate the effectiveness of UW solution in preservation of the viability of ADSCs. We collected adipose tissue from the inguinal fat pad of mice and compared preservation in UW solution and HBSS overnight by measuring cell viability after isolation. We found that the number of viable cells harvested per gram of adipose tissue mass was higher in UW solution-than HBSS-preserved tissue.
AB - Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank's balanced salt solution (HBSS) is one of the main ADSC preservation solutions used clinically. However, this step is known to lead to decreased cell viability. The University of Wisconsin (UW) solution is recognized by transplant physicians as an excellent organ preservation solution. We aimed to investigate the effectiveness of UW solution in preservation of the viability of ADSCs. We collected adipose tissue from the inguinal fat pad of mice and compared preservation in UW solution and HBSS overnight by measuring cell viability after isolation. We found that the number of viable cells harvested per gram of adipose tissue mass was higher in UW solution-than HBSS-preserved tissue.
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U2 - 10.1155/2018/1625464
DO - 10.1155/2018/1625464
M3 - Article
AN - SCOPUS:85057288103
VL - 2018
JO - Stem Cells International
JF - Stem Cells International
SN - 1687-9678
M1 - 1625464
ER -