A comparison of heat shock protein genes from cultured cells of the cabbage armyworm, Mamestra brassicae, in response to heavy metals

Shoji Sonoda, Muhammad Ashfaq, Hisaaki Tsumuki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Heat shock protein (HSP) genes, hsp90, hsp70, hsc70, hsp20.7, and hsp19.7, were cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. Analyses of the cDNA sequences revealed open reading frames of 2,151, 1,914, 1,962, 540, and 465 bp in lengths, which encode proteins with calculated molecular weights of 82.5, 69.9, 71.6, 20.7, and 19.7 kDa, respectively. An increased expression was observed in all five genes after exposure to a high temperature. The induction of gene expression was not observed during a low temperature exposure, but was observed when the cells were recovered at ambient temperature. Expression of hsp90, hsp70, and hsp20.7 was induced after exposure to 2 μM of cadmium, while the minimum cadmium concentration for induction of hsp19.7 was 5 μM. The induction of hsp90 expression was somewhat masked by basal levels of expression. Only hsp20.7 expression was induced by exposure to copper. Lead did not induce expression of any of the HSP genes tested. Cadmium-induced up-regulation of hsp70 expression was lasted longer than heat-induced one. These results suggest that hsp70 could be useful to assess the cellular distress or injury induced by cadmium.

Original languageEnglish
Pages (from-to)210-222
Number of pages13
JournalArchives of Insect Biochemistry and Physiology
Volume65
Issue number4
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Mamestra brassicae
Brassica
Heat-Shock Proteins
Heavy Metals
Cadmium
heat shock proteins
cabbage
cultured cells
cadmium
Cultured Cells
heavy metals
Genes
Cells
Temperature
genes
distress
Gene expression
Open Reading Frames
Sequence Analysis
open reading frames

Keywords

  • Biomarker
  • Cabbage armyworm
  • Heat shock protein
  • Heavy metal
  • Mamestra brassicae

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Cite this

A comparison of heat shock protein genes from cultured cells of the cabbage armyworm, Mamestra brassicae, in response to heavy metals. / Sonoda, Shoji; Ashfaq, Muhammad; Tsumuki, Hisaaki.

In: Archives of Insect Biochemistry and Physiology, Vol. 65, No. 4, 08.2007, p. 210-222.

Research output: Contribution to journalArticle

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