A combined light and scanning electron microscopy on human urinary cytology

Hiromi Kumon1; Yasutomo Nasu; Yosuke Matsumura; Hiroyuki Ohmori; Toshio Tanaka

doi:10.1093/oxfordjournals.jmicro.a050392

A combined light and scanning electron microscopy on human urinary cytology

Hiromi Kumon1, Yasutomo Nasu, Yosuke Matsumura, Hiroyuki Ohmori, Toshio Tanaka

Okayama University

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

Approximately 250 bladder-washing and voided urine specimens from normal, and inflammatory and malignant lesions were examined using light microscopy (LM), scanning electron microscopy (SEM) and a newly-designed mesh. This specific mesh, containing 42 compartments in 25 mm2 on gelatine- coated glass-slip, permitted us the direct correlation of LM and SEM images. With SEM, malignancy of exfoliated urothelial cells mainly depended on the presence or absence of pleomorphic microvilli. Subsequently, a new “SEM grading” regarding human urinary cytology was proposed.

Original language	English
Pages (from-to)	27-32
Number of pages	6
Journal	Microscopy
Volume	32
Issue number	1
DOIs	https://doi.org/10.1093/oxfordjournals.jmicro.a050392
Publication status	Published - Jan 1 1983

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Keywords

Human bladder cancer
Pleomorphic microvilli
Scanning electron microscopy (SEM)
Urinary cytology

ASJC Scopus subject areas

Structural Biology
Instrumentation
Radiology Nuclear Medicine and imaging

Cite this

Kumon1, H., Nasu, Y., Matsumura, Y., Ohmori, H., & Tanaka, T. (1983). A combined light and scanning electron microscopy on human urinary cytology. Microscopy, 32(1), 27-32. https://doi.org/10.1093/oxfordjournals.jmicro.a050392

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10.1093/oxfordjournals.jmicro.a050392