A combined light and scanning electron microscopy on human urinary cytology

Hiromi Kumon1, Yasutomo Nasu, Yosuke Matsumura, Hiroyuki Ohmori, Toshio Tanaka

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)


    Approximately 250 bladder-washing and voided urine specimens from normal, and inflammatory and malignant lesions were examined using light microscopy (LM), scanning electron microscopy (SEM) and a newly-designed mesh. This specific mesh, containing 42 compartments in 25 mm2 on gelatine- coated glass-slip, permitted us the direct correlation of LM and SEM images. With SEM, malignancy of exfoliated urothelial cells mainly depended on the presence or absence of pleomorphic microvilli. Subsequently, a new “SEM grading” regarding human urinary cytology was proposed.

    Original languageEnglish
    Pages (from-to)27-32
    Number of pages6
    Issue number1
    Publication statusPublished - 1983


    • Human bladder cancer
    • Pleomorphic microvilli
    • Scanning electron microscopy (SEM)
    • Urinary cytology

    ASJC Scopus subject areas

    • Structural Biology
    • Instrumentation
    • Radiology Nuclear Medicine and imaging


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