A combination of roxithromycin and imipenem as an antimicrobial strategy against biofilms formed by Staphylococcus aureus

Osamu Yamasaki, H. Akiyama, Y. Toi, J. Arata

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We examined the effects of a combination of roxithromycin and imipenem on a biofilm model of Staphylococcus aureus. Treatment with roxithromycin alone and imipenem alone did not decrease the number of viable bacterial cells compared with the control. However, a combination treatment of roxithromycin and imipenem significantly decreased the number of viable bacterial cells on day 8 after inoculation in the in vivo model (P <0.01). On days 5 and 8 after inoculation, numerous polymorphonuclear leucocytes and macrophages invaded the bacterial clusters in the roxithromycin- and roxithromycin/imipenem-treated groups, but did not invade the control or imipenem-treated groups. The present study indicated that a combination of roxithromycin and imipenem is a potentially effective treatment for S. aureus biofilm-associated skin infections as it can induce the invasion of polymorphonuclear leucocytes into the biofilm.

Original languageEnglish
Pages (from-to)573-577
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume48
Issue number4
Publication statusPublished - 2001

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Roxithromycin
Imipenem
Biofilms
Staphylococcus aureus
Neutrophils
Macrophages
Skin
Infection

ASJC Scopus subject areas

  • Microbiology
  • Pharmacology

Cite this

A combination of roxithromycin and imipenem as an antimicrobial strategy against biofilms formed by Staphylococcus aureus. / Yamasaki, Osamu; Akiyama, H.; Toi, Y.; Arata, J.

In: Journal of Antimicrobial Chemotherapy, Vol. 48, No. 4, 2001, p. 573-577.

Research output: Contribution to journalArticle

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