A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides

Rumiko Shimizu, Ai Nagai, Hiroko Tominaga, Megumi Imura, Arnold N. Onyango, Minoru Izumi, Shuhei Nakajima, Shoichi Tahara, Takao Kaneko, Naomichi Baba

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2 Citations (Scopus)


An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.

Original languageEnglish
Pages (from-to)781-784
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2009



  • ESI MS
  • Lipid hydroperoxide
  • Molecular probe
  • Phosphatidylcholine hydroperoxide
  • Tandem ESI MS

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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