A combination of molecular probe-tandem electrospray ionization mass spectrometry: A technique for tracing structural changes in phospholipid hydroperoxides

Rumiko Shimizu, Ai Nagai, Hiroko Tominaga, Megumi Imura, Arnold N. Onyango, Minoru Izumi, Shuhei Nakajima, Shoichi Tahara, Takao Kaneko, Naomichi Baba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.

Original languageEnglish
Pages (from-to)781-784
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number3
DOIs
Publication statusPublished - 2009

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Keywords

  • ESI MS
  • Lipid hydroperoxide
  • Molecular probe
  • Phosphatidylcholine hydroperoxide
  • Tandem ESI MS

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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