A combination of five mechanisms confers a high tolerance for aluminum to a wild species of Poaceae, Andropogon virginicus L.

Bunichi Ezaki, Kottapalli Jayaram, Aiko Higashi, Kenkou Takahashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

How can high tolerance against aluminum (Al) toxicity be obtained in plants? To address this question, tolerant mechanisms were characterized in a highly Al tolerant wild species of Poaceae, Andropogon virginicus L. A. virginicus showed an Al-stress-induced synthesis and secretion of citrate and malate in roots. This mechanism may help to suppress an increase of toxic Al ions in the root region. Microscopic observation of the morin-stained leaves indicated that the Al transferred to shoots was specifically accumulated in the trichomes and spikes of the leaves and that some portion of the accumulated Al was furthermore secreted as sap from the tips of trichomes. Al-induced synthesis of poly-phenolic compounds including anthocyanin also occurred in roots as a long term response to Al toxicity and anthocyanin production did not co-localize with either Al accumulation, nitric oxide (NO) production or lipid peroxides production in the roots. It was suggested that oxidative damage caused by Al stress was suppressed in these areas where anthocyanin was localized. Moreover, induction of NO production occurred in roots within 24. h of Al treatment. Our results suggested that NO could not efficiently ameliorate the Al-dependent nuclei deformation and DNA fragmentation, but could function as a trigger to stimulate anti-peroxidation enzymes under Al stress. Collectively the results suggested that A. virginicus manifests its high Al tolerance by a unique combination of effective mechanisms.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalEnvironmental and Experimental Botany
Volume93
DOIs
Publication statusPublished - Sep 2013

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Andropogon virginicus
Poaceae
aluminum
tolerance
nitric oxide
anthocyanins
trichome
trichomes
toxicity
synthesis

Keywords

  • Aluminum (Al) stress
  • Anthocyanin
  • Nitric oxide (NO)
  • Organic acid
  • Oxidative stress
  • Trichome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Agronomy and Crop Science

Cite this

A combination of five mechanisms confers a high tolerance for aluminum to a wild species of Poaceae, Andropogon virginicus L. / Ezaki, Bunichi; Jayaram, Kottapalli; Higashi, Aiko; Takahashi, Kenkou.

In: Environmental and Experimental Botany, Vol. 93, 09.2013, p. 35-44.

Research output: Contribution to journalArticle

Ezaki, Bunichi ; Jayaram, Kottapalli ; Higashi, Aiko ; Takahashi, Kenkou. / A combination of five mechanisms confers a high tolerance for aluminum to a wild species of Poaceae, Andropogon virginicus L. In: Environmental and Experimental Botany. 2013 ; Vol. 93. pp. 35-44.
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