A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria: A field trial in the Solomon Islands

Meiji Arai, Kaori Kunisada, Hye-Sook Kim, Hirofumi Miyake, Chiyoko Mizukoshi, Toshifumi Kakutani, Akio Yamane, Satoru Nakagami, Shintaro Kawai, Hiromi Nakano, Fumihiko Kawamoto, Yusuke Wataya

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have developed a colorimetric assay, 'microtiter plate-hybridization', for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, in which the target DNA sequences (18S small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with the species-specific probes immobilized on a microtiter well. This assay system was tested in Guadalcanal, Solomon Islands, where malaria is highly endemic. We obtained blood samples by finger puncture from 130 asymptomatic donors. Among the 130 samples, 30 (23%) were P. falciparum positive, 28 (22%) were P. vivax positive, and 8 (6%) were mixed infections. The results of our DNA diagnostic method showed good correlation with those of acridine orange microscopy.

Original languageEnglish
Pages (from-to)719-731
Number of pages13
JournalNucleosides and Nucleotides
Volume15
Issue number1-3
Publication statusPublished - 1996

Fingerprint

Melanesia
Vivax Malaria
Falciparum Malaria
Assays
Blood
Acridine Orange
Ribosomal RNA
Polymerase chain reaction
DNA sequences
DNA
Plasmodium falciparum
Coinfection
rRNA Genes
Punctures
Malaria
Fingers
Microscopy
Microscopic examination
Parasites
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Arai, M., Kunisada, K., Kim, H-S., Miyake, H., Mizukoshi, C., Kakutani, T., ... Wataya, Y. (1996). A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria: A field trial in the Solomon Islands. Nucleosides and Nucleotides, 15(1-3), 719-731.

A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria : A field trial in the Solomon Islands. / Arai, Meiji; Kunisada, Kaori; Kim, Hye-Sook; Miyake, Hirofumi; Mizukoshi, Chiyoko; Kakutani, Toshifumi; Yamane, Akio; Nakagami, Satoru; Kawai, Shintaro; Nakano, Hiromi; Kawamoto, Fumihiko; Wataya, Yusuke.

In: Nucleosides and Nucleotides, Vol. 15, No. 1-3, 1996, p. 719-731.

Research output: Contribution to journalArticle

Arai, M, Kunisada, K, Kim, H-S, Miyake, H, Mizukoshi, C, Kakutani, T, Yamane, A, Nakagami, S, Kawai, S, Nakano, H, Kawamoto, F & Wataya, Y 1996, 'A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria: A field trial in the Solomon Islands', Nucleosides and Nucleotides, vol. 15, no. 1-3, pp. 719-731.
Arai, Meiji ; Kunisada, Kaori ; Kim, Hye-Sook ; Miyake, Hirofumi ; Mizukoshi, Chiyoko ; Kakutani, Toshifumi ; Yamane, Akio ; Nakagami, Satoru ; Kawai, Shintaro ; Nakano, Hiromi ; Kawamoto, Fumihiko ; Wataya, Yusuke. / A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria : A field trial in the Solomon Islands. In: Nucleosides and Nucleotides. 1996 ; Vol. 15, No. 1-3. pp. 719-731.
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