TY - JOUR
T1 - A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria
T2 - A field trial in the Solomon Islands
AU - Arai, Meiji
AU - Kunisada, Kaori
AU - Kim, Hye Sook
AU - Miyake, Hirofumi
AU - Mizukoshi, Chiyoko
AU - Kakutani, Toshifumi
AU - Yamane, Akio
AU - Nakagami, Satoru
AU - Kawai, Shintaro
AU - Nakano, Hiromi
AU - Kawamoto, Fumihiko
AU - Wataya, Yusuke
PY - 1996
Y1 - 1996
N2 - We have developed a colorimetric assay, 'microtiter plate-hybridization', for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, in which the target DNA sequences (18S small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with the species-specific probes immobilized on a microtiter well. This assay system was tested in Guadalcanal, Solomon Islands, where malaria is highly endemic. We obtained blood samples by finger puncture from 130 asymptomatic donors. Among the 130 samples, 30 (23%) were P. falciparum positive, 28 (22%) were P. vivax positive, and 8 (6%) were mixed infections. The results of our DNA diagnostic method showed good correlation with those of acridine orange microscopy.
AB - We have developed a colorimetric assay, 'microtiter plate-hybridization', for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, in which the target DNA sequences (18S small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with the species-specific probes immobilized on a microtiter well. This assay system was tested in Guadalcanal, Solomon Islands, where malaria is highly endemic. We obtained blood samples by finger puncture from 130 asymptomatic donors. Among the 130 samples, 30 (23%) were P. falciparum positive, 28 (22%) were P. vivax positive, and 8 (6%) were mixed infections. The results of our DNA diagnostic method showed good correlation with those of acridine orange microscopy.
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U2 - 10.1080/07328319608002417
DO - 10.1080/07328319608002417
M3 - Article
AN - SCOPUS:9044251600
SN - 0732-8311
VL - 15
SP - 719
EP - 731
JO - Nucleosides and Nucleotides
JF - Nucleosides and Nucleotides
IS - 1-3
ER -
