A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized In Vivo

Fuminari Uehara, Yasunori Tome, Shuya Yano, Shinji Miwa, Sumiyuki Mii, Yukihiko Hiroshima, Michael Bouvet, Hiroki Maehara, Fuminori Kanaya, Robert M. Hoffman

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11 Citations (Scopus)

Abstract

The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam® vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing αv integrin-GFP were generated by transfection with an αv integrin-GFP vector. Gelfoam® (5×5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam® became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam® for observing vascularization of the Gelfoam® using fluorescence imaging. Gelfoam® is a useful tool to observe angiogenesis in vivo. 143B cells (5×105) expressing αv integrin-GFP were injected into the Gelfoam® seven days after transplantation of Gelfoam®. Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam® by color-coded confocal microscopy via the skin flap. The 143B cells expressing αv integrin-GFP proliferated into the Gelfoam®, which contained RFP-expressing blood vessels. Strong expression of αv integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam®. The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells.

Original languageEnglish
Pages (from-to)1361-1366
Number of pages6
JournalAnticancer research
Volume33
Issue number4
Publication statusPublished - Apr 2013

Keywords

  • Blood vessels
  • Confocal microscopy
  • Gelfoam®
  • Green fluorescent protein
  • Osteosarcoma
  • Red fluorescent protein
  • Transgenic nude mouse
  • α integrin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Uehara, F., Tome, Y., Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Bouvet, M., Maehara, H., Kanaya, F., & Hoffman, R. M. (2013). A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized In Vivo. Anticancer research, 33(4), 1361-1366.