A Clostridium perfringens vector for the selection of promoters

Chieko Matsushita, Osamu Matsushita, Michio Koyama, Akjnobu Okabe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A promoter selection vector for Clostridium perfringens genes was constructed from a C perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be a useful reporter system for C. perfringens genes.

Original languageEnglish
Pages (from-to)317-319
Number of pages3
JournalPlasmid
Volume31
Issue number3
DOIs
Publication statusPublished - May 1994
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

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