A Clostridium perfringens Vector for the Selection of Promoters

Chieko Matsushita, Osamu Matsushita, Michio Koyama, Akinobu Okabe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A promoter selection vector for Clostridium perfringens genes was constructed from a C. perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be a useful reporter system for C. perfringens genes.

Original languageEnglish
Pages (from-to)317-319
Number of pages3
JournalPlasmid
Volume31
Issue number3
DOIs
Publication statusPublished - May 1994
Externally publishedYes

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Clostridium perfringens
Plasmids
Genes
Organism Cloning
Chloramphenicol Resistance
Genetic Vectors
Chloramphenicol O-Acetyltransferase
Type C Phospholipases
Genetic Promoter Regions
Escherichia coli

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A Clostridium perfringens Vector for the Selection of Promoters. / Matsushita, Chieko; Matsushita, Osamu; Koyama, Michio; Okabe, Akinobu.

In: Plasmid, Vol. 31, No. 3, 05.1994, p. 317-319.

Research output: Contribution to journalArticle

Matsushita, Chieko ; Matsushita, Osamu ; Koyama, Michio ; Okabe, Akinobu. / A Clostridium perfringens Vector for the Selection of Promoters. In: Plasmid. 1994 ; Vol. 31, No. 3. pp. 317-319.
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