A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis

Michio Koyama, Seiichi Katayama, Masato Kaji, Yuki Taniguchi, Osamu Matsushita, Junzaburo Minami, Shushi Morita, Akinobu Okabe

Research output: Contribution to journalArticle

Abstract

The hem gene cluster, which consists of hemA, cysG(B), hemC, hemD, hemB, and hemL genes, and encodes enzymes involved in the biosynthetic pathway from glutamyl-tRNA to uroporphyrinogen III, has been identified by the cloning and sequencing of two overlapping DNA fragments from Clostridium perfringens NCTC8237. The deduced amino acid sequence of the N-terminal region of C. perfringens HemD is homologous to those reported for the C-terminal region of Salmonella typhimurium CysG and Clostridium josui HemD. C. perfringens CysG(β) is a predicted 220-residue protein which shows homology to the N- terminal region of S. typhimurium CysG. Disruption of the cysG(β) gene in C. perfringens strain 13 by homologous recombination reduced cobalamin (vitamin B12 levels by a factor of 200. When grown in vitamin B12-deficient medium, the mutant strain showed a four-fold increase in its doubling time compared with that of the wild-type strain, and this effect was counteracted by supplementing the medium with vitamin B12. These results suggest that C. perfringens CysG(β) is involved in the chelation of cobalt to precorrin II as suggested for the CysG(β) domain of S. typhimurium CysG, enabling the synthesis of cobalamin.

Original languageEnglish
Pages (from-to)947-957
Number of pages11
JournalMicrobiology and Immunology
Volume43
Issue number10
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Clostridium perfringens
Vitamin B 12
Multigene Family
Salmonella typhimurium
Uroporphyrinogens
Clostridium
Homologous Recombination
Biosynthetic Pathways
Transfer RNA
Cobalt
Genes
Organism Cloning
Amino Acid Sequence
DNA
Enzymes
Proteins

Keywords

  • Clostridium perfringens
  • Cobalamin biosynthesis
  • Hem genes
  • Tetrapyrrole

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Koyama, M., Katayama, S., Kaji, M., Taniguchi, Y., Matsushita, O., Minami, J., ... Okabe, A. (1999). A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis. Microbiology and Immunology, 43(10), 947-957.

A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis. / Koyama, Michio; Katayama, Seiichi; Kaji, Masato; Taniguchi, Yuki; Matsushita, Osamu; Minami, Junzaburo; Morita, Shushi; Okabe, Akinobu.

In: Microbiology and Immunology, Vol. 43, No. 10, 1999, p. 947-957.

Research output: Contribution to journalArticle

Koyama, M, Katayama, S, Kaji, M, Taniguchi, Y, Matsushita, O, Minami, J, Morita, S & Okabe, A 1999, 'A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis', Microbiology and Immunology, vol. 43, no. 10, pp. 947-957.
Koyama, Michio ; Katayama, Seiichi ; Kaji, Masato ; Taniguchi, Yuki ; Matsushita, Osamu ; Minami, Junzaburo ; Morita, Shushi ; Okabe, Akinobu. / A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis. In: Microbiology and Immunology. 1999 ; Vol. 43, No. 10. pp. 947-957.
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