A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor

Masaharu Takigawa, H. O. Pan, M. Enomoto, A. Kinoshita, Y. Nishida, F. Suzuki, K. Tajima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Previously we reported that rabbit costal chondrocytes in primary culture produced an anti-angiogenic antitumor factor. We also established a clonal human chondrosarcoma cell line, HCS-2/8, which had typical cartilage phenotypes. In the present study, we investigated whether HCS-2/8 cells produce an anti-angiogenic anti-tumor factor. Serum-free medium conditioned by exposure to HCS-2/8 cells in culture inhibited the proliferation of bovine pulmonary artery endothelial cells in culture. The inhibition was not due to general cytotoxicity because the endothelial cells started to proliferate after the conditioned medium was removed from the cultures. The factor in conditioned medium also inhibited DNA synthesis in bovine pulmonary artery endothelial cells, but not in B16 melanoma cells, normal rat kidney cells or chick embryo fibroblasts. Production of the factor by HCS-2/8 cells did not change during serial passages. It also inhibited angiogenesis in the chorioallantoic membrane of chick embryos induced by B16 melanoma and growth of the tumor transplanted onto the membrane. Treatment with protease destroyed the inhibitory activities of conditioned medium on angiogenesis and tumor growth. These findings suggest that HCS-2/8 cells produce a peptide factor with anti-angiogenic, antitumor activity and that the cell line should be a useful source of the factor.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalAnticancer Research
Volume10
Issue number2 A
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Chondrosarcoma
Angiogenesis Inducing Agents
Cell Line
Conditioned Culture Medium
Experimental Melanomas
Endothelial Cells
Chick Embryo
Pulmonary Artery
Cell Culture Techniques
Serial Passage
Chorioallantoic Membrane
Serum-Free Culture Media
Chondrocytes
Growth
Cartilage
Neoplasms
Peptide Hydrolases
Fibroblasts
Rabbits
Phenotype

Keywords

  • anti-angiogenesis factor
  • antitumor factor
  • cell line
  • chondrocyte
  • chondrosarcoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Takigawa, M., Pan, H. O., Enomoto, M., Kinoshita, A., Nishida, Y., Suzuki, F., & Tajima, K. (1990). A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor. Anticancer Research, 10(2 A), 311-315.

A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor. / Takigawa, Masaharu; Pan, H. O.; Enomoto, M.; Kinoshita, A.; Nishida, Y.; Suzuki, F.; Tajima, K.

In: Anticancer Research, Vol. 10, No. 2 A, 1990, p. 311-315.

Research output: Contribution to journalArticle

Takigawa, M, Pan, HO, Enomoto, M, Kinoshita, A, Nishida, Y, Suzuki, F & Tajima, K 1990, 'A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor', Anticancer Research, vol. 10, no. 2 A, pp. 311-315.
Takigawa M, Pan HO, Enomoto M, Kinoshita A, Nishida Y, Suzuki F et al. A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor. Anticancer Research. 1990;10(2 A):311-315.
Takigawa, Masaharu ; Pan, H. O. ; Enomoto, M. ; Kinoshita, A. ; Nishida, Y. ; Suzuki, F. ; Tajima, K. / A clonal human chondrosarcoma cell line produces an anti-angiogenic antitumor factor. In: Anticancer Research. 1990 ; Vol. 10, No. 2 A. pp. 311-315.
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