A chick wingless mutation causes abnormality in maintenance of Fgf8 expression in the wing apical ridge, resulting in loss of the dorsoventral boundary

Hideyo Ohuchi, Mami Shibusawa, Takashi Nakagawa, Takeshi Ohata, Hidefumi Yoshioka, Yasokazu Hirai, Tsutomu Nohno, Sumihare Noji, Norio Kondo

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We analyzed a Japanese chick wingless mutant (Jwg) to know a molecular mechanism underlying wing development. We observed expression patterns of eleven marker genes to characterize the mutant. Expressions of dorsoventral (DV) and mesenchymal marker genes were intact in nascent Jwg limb buds. However, expression of Fgf8, a marker gene for the apical ectodermal ridge (AER), was delayed and shortly disappeared in the wing regressing AER. Later on, ventral expression of dorsal marker genes of Wnt7a and Lmx1 indicated that the wing bud without the AER became bi-dorsal. In addition, the posterior mesoderm became defective, as deduced from the impaired expression patterns of Sonic hedgehog (Shh), Msx1, and Prx1. We attempted to rescue a wing by implanting Fgf8-expressing cells into the Jwg wing bud. We found that FGF8 can rescue outgrowth of the wing bud by maintaining Shh expression. Thus, the Jwg gene seems to be involved in maintenance of the Fgf8 expression in the wing bud. Further, it is suggested that the AER is required for maintenance of the DV boundary and the polarizing activity of the established wing bud.

Original languageEnglish
Pages (from-to)3-13
Number of pages11
JournalMechanisms of Development
Volume62
Issue number1
DOIs
Publication statusPublished - Feb 1997
Externally publishedYes

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Maintenance
Mutation
Genes
Limb Buds
Mesoderm

Keywords

  • apical ectodermal ridge
  • chick
  • dorsoventral boundary
  • Fgf8
  • limb development
  • wingless mutant

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

A chick wingless mutation causes abnormality in maintenance of Fgf8 expression in the wing apical ridge, resulting in loss of the dorsoventral boundary. / Ohuchi, Hideyo; Shibusawa, Mami; Nakagawa, Takashi; Ohata, Takeshi; Yoshioka, Hidefumi; Hirai, Yasokazu; Nohno, Tsutomu; Noji, Sumihare; Kondo, Norio.

In: Mechanisms of Development, Vol. 62, No. 1, 02.1997, p. 3-13.

Research output: Contribution to journalArticle

Ohuchi, Hideyo ; Shibusawa, Mami ; Nakagawa, Takashi ; Ohata, Takeshi ; Yoshioka, Hidefumi ; Hirai, Yasokazu ; Nohno, Tsutomu ; Noji, Sumihare ; Kondo, Norio. / A chick wingless mutation causes abnormality in maintenance of Fgf8 expression in the wing apical ridge, resulting in loss of the dorsoventral boundary. In: Mechanisms of Development. 1997 ; Vol. 62, No. 1. pp. 3-13.
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