A centromeric tandem repeat family originating from a part of Ty3/gypsy-retroelement in wheat and its relatives

Zhi Jun Cheng, Minoru Murata

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

From a wild diploid species that is a relative of wheat, Aegilops speltoides, a 301-bp repeat containing 16 copies of a CAA microsatellite was isolated. Southern blot and fluorescence in situ hybridization revealed that ∼250 bp of the sequence is tandemly arrayed at the centromere regions of A- and B-genome chromosomes of common wheat and rye chromosomes. Although the DNA sequence of this 250-bp repeat showed no notable homology in the databases, the flanking or intervening sequences between the repeats showed high homologies (>82%) to two separate sequences of the gag gene and its upstream region in cereba, a Ty3/gypsy-like retroelement of Hordeum vulgare. Since the amino acid sequence deduced from the 250 bp with seven CAAs showed some similarity (∼53%) to that of the gag gene, we concluded that the 250-bp repeats had also originated from the cereba-like retroelements in diploid wheat such as Ae. speltoides and had formed tandem arrays, whereas the 300-bp repeats were dispersed as a part of cereba-like retroelements. This suggests that some tandem repeats localized at the centromeric regions of cereals and other plant species originated from parts of retrotransposons.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalGenetics
Volume164
Issue number2
Publication statusPublished - Jun 1 2003

Fingerprint

Retroelements
Tandem Repeat Sequences
Triticum
gag Genes
Diploidy
Chromosomes, Human, 4-5
Centromere
Hordeum
Southern Blotting
Fluorescence In Situ Hybridization
Microsatellite Repeats
Introns
Amino Acid Sequence
Chromosomes
Genome
Databases

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

A centromeric tandem repeat family originating from a part of Ty3/gypsy-retroelement in wheat and its relatives. / Cheng, Zhi Jun; Murata, Minoru.

In: Genetics, Vol. 164, No. 2, 01.06.2003, p. 665-672.

Research output: Contribution to journalArticle

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