A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco

Kiyotaka Nagaki, Kazunari Kashihara, Minoru Murata

    Research output: Contribution to journalArticlepeer-review

    38 Citations (Scopus)

    Abstract

    Centromeres play an important role in segregating chromosomes into daughter cells, and centromeric DNA assembles specific proteins to form a complex referred to as the kinetochore. Among these proteins, centromere-specific histone H3 (CENH3) is one of the most characterized and found to be located only on active centromeres. We isolated four different CENH3-coding complementary DNAs (cDNAs), two from Nicotiana tabaccum and one each from the ancestral diploid species, Nicotiana sylvestris and Nicotiana tomentosiformis and raised an antibody against N-terminal amino acid sequences deduced from the cDNAs. Immunostaining with the antibody revealed the preferential centromere localization, indicating that the cDNAs cloned in this study encode authentic tobacco CENH3. A tobacco centromeric DNA sequence (Nt2-7) was also identified by chromatin immunoprecipitation cloning using the antibody.

    Original languageEnglish
    Pages (from-to)249-257
    Number of pages9
    JournalChromosoma
    Volume118
    Issue number2
    DOIs
    Publication statusPublished - 2009

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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