A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco

Kiyotaka Nagaki, Kazunari Kashihara, Minoru Murata

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Centromeres play an important role in segregating chromosomes into daughter cells, and centromeric DNA assembles specific proteins to form a complex referred to as the kinetochore. Among these proteins, centromere-specific histone H3 (CENH3) is one of the most characterized and found to be located only on active centromeres. We isolated four different CENH3-coding complementary DNAs (cDNAs), two from Nicotiana tabaccum and one each from the ancestral diploid species, Nicotiana sylvestris and Nicotiana tomentosiformis and raised an antibody against N-terminal amino acid sequences deduced from the cDNAs. Immunostaining with the antibody revealed the preferential centromere localization, indicating that the cDNAs cloned in this study encode authentic tobacco CENH3. A tobacco centromeric DNA sequence (Nt2-7) was also identified by chromatin immunoprecipitation cloning using the antibody.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalChromosoma
Volume118
Issue number2
DOIs
Publication statusPublished - 2009

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Centromere
Histones
Tobacco
Complementary DNA
Antibodies
Kinetochores
Chromatin Immunoprecipitation
Diploidy
Organism Cloning
Amino Acid Sequence
Proteins
Chromosomes
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco. / Nagaki, Kiyotaka; Kashihara, Kazunari; Murata, Minoru.

In: Chromosoma, Vol. 118, No. 2, 2009, p. 249-257.

Research output: Contribution to journalArticle

Nagaki, Kiyotaka ; Kashihara, Kazunari ; Murata, Minoru. / A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco. In: Chromosoma. 2009 ; Vol. 118, No. 2. pp. 249-257.
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