A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

Shoko Ueki, Benjamin L. Meyers, Farzana Yasmin, Vitaly Citovsky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

Original languageEnglish
Article numbere2208
JournalJournal of visualized experiments : JoVE
Issue number42
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Fingerprint

Biolistics
Tobacco
Assays
Proteins
Protein Transport
Arabidopsis
Confocal Microscopy
Confocal microscopy
Technology
DNA
Tissue

Keywords

  • Cellular Biology
  • Confocal microscopy
  • Fluorescent protein
  • Issue 42
  • Microbombardment
  • Plant
  • Symplastic transport
  • Transient expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment. / Ueki, Shoko; Meyers, Benjamin L.; Yasmin, Farzana; Citovsky, Vitaly.

In: Journal of visualized experiments : JoVE, No. 42, e2208, 08.2010.

Research output: Contribution to journalArticle

@article{6ce4e488e06944bb9eb0b65fec7549e3,
title = "A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment",
abstract = "Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).",
keywords = "Cellular Biology, Confocal microscopy, Fluorescent protein, Issue 42, Microbombardment, Plant, Symplastic transport, Transient expression",
author = "Shoko Ueki and Meyers, {Benjamin L.} and Farzana Yasmin and Vitaly Citovsky",
year = "2010",
month = "8",
doi = "10.3791/2208",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "42",

}

TY - JOUR

T1 - A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

AU - Ueki, Shoko

AU - Meyers, Benjamin L.

AU - Yasmin, Farzana

AU - Citovsky, Vitaly

PY - 2010/8

Y1 - 2010/8

N2 - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

AB - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

KW - Cellular Biology

KW - Confocal microscopy

KW - Fluorescent protein

KW - Issue 42

KW - Microbombardment

KW - Plant

KW - Symplastic transport

KW - Transient expression

UR - http://www.scopus.com/inward/record.url?scp=80055116758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80055116758&partnerID=8YFLogxK

U2 - 10.3791/2208

DO - 10.3791/2208

M3 - Article

C2 - 20834216

AN - SCOPUS:80055116758

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 42

M1 - e2208

ER -