Abstract
Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).
| Original language | English |
|---|---|
| Article number | e2208 |
| Journal | Journal of visualized experiments : JoVE |
| Issue number | 42 |
| DOIs | |
| Publication status | Published - Aug 2010 |
| Externally published | Yes |
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Keywords
- Cellular Biology
- Confocal microscopy
- Fluorescent protein
- Issue 42
- Microbombardment
- Plant
- Symplastic transport
- Transient expression
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Chemical Engineering(all)
- Immunology and Microbiology(all)
- Neuroscience(all)
Cite this
A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment. / Ueki, Shoko; Meyers, Benjamin L.; Yasmin, Farzana; Citovsky, Vitaly.
In: Journal of visualized experiments : JoVE, No. 42, e2208, 08.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment
AU - Ueki, Shoko
AU - Meyers, Benjamin L.
AU - Yasmin, Farzana
AU - Citovsky, Vitaly
PY - 2010/8
Y1 - 2010/8
N2 - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).
AB - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).
KW - Cellular Biology
KW - Confocal microscopy
KW - Fluorescent protein
KW - Issue 42
KW - Microbombardment
KW - Plant
KW - Symplastic transport
KW - Transient expression
UR - http://www.scopus.com/inward/record.url?scp=80055116758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80055116758&partnerID=8YFLogxK
U2 - 10.3791/2208
DO - 10.3791/2208
M3 - Article
C2 - 20834216
AN - SCOPUS:80055116758
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 42
M1 - e2208
ER -