A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

Shoko Ueki; Benjamin L. Meyers; Farzana Yasmin; Vitaly Citovsky

doi:10.3791/2208

A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

Shoko Ueki, Benjamin L. Meyers, Farzana Yasmin, Vitaly Citovsky

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

Original language	English
Article number	e2208
Journal	Journal of Visualized Experiments
Issue number	42
DOIs	https://doi.org/10.3791/2208
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

Cellular Biology
Confocal microscopy
Fluorescent protein
Issue 42
Microbombardment
Plant
Symplastic transport
Transient expression

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.3791/2208

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title = "A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment",

abstract = "Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).",

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T1 - A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

AU - Ueki, Shoko

AU - Meyers, Benjamin L.

AU - Yasmin, Farzana

AU - Citovsky, Vitaly

PY - 2010/8

Y1 - 2010/8

N2 - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

AB - Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

KW - Cellular Biology

KW - Confocal microscopy

KW - Fluorescent protein

KW - Issue 42

KW - Microbombardment

KW - Plant

KW - Symplastic transport

KW - Transient expression

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SN - 1940-087X

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ER -

A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

Abstract

Keywords

ASJC Scopus subject areas

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