A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment

Shoko Ueki, Benjamin L. Meyers, Farzana Yasmin, Vitaly Citovsky

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).

Original languageEnglish
Article numbere2208
JournalJournal of Visualized Experiments
Issue number42
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Keywords

  • Cellular Biology
  • Confocal microscopy
  • Fluorescent protein
  • Issue 42
  • Microbombardment
  • Plant
  • Symplastic transport
  • Transient expression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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