Abstract
Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).
Original language | English |
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Article number | e2208 |
Journal | Journal of Visualized Experiments |
Issue number | 42 |
DOIs | |
Publication status | Published - Aug 2010 |
Externally published | Yes |
Keywords
- Cellular Biology
- Confocal microscopy
- Fluorescent protein
- Issue 42
- Microbombardment
- Plant
- Symplastic transport
- Transient expression
ASJC Scopus subject areas
- Neuroscience(all)
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)