A cdc5+ homolog of a higher plant, Arabidopsis thaliana

Takashi Hirayama, Kazuo Shinozaki

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We cloned and characterized a cDNA corresponding to a cdc5+ homolog of the higher plant, Arabidopsis thaliana. The cDNA, named AtCDC5 cDNA, encodes a polypeptide of 844 amino acid residues. The amino acid sequence of N- terminal one-fourth region of the predicted protein bears significant similarity to that of Schizosaccharomyces pombe Cdc5 and Myb-related proteins. Overexpression of the AtCDC5 cDNA in S. pombe cells is able to complement the growth defective phenotype of a cdc5 temperature-sensitive mutant. These results indicate that the AtCDC5 gene is a plant counterpart of S. pombe cdc5+. This is the first report of a cdc5+-like gene in a multicellular organism. We also demonstrated that a recombinant AtCDC5 protein possesses a sequence specific DNA binding activity (CTCAGCG) and the AtCDC5 gene is expressed extensively in shoot and root meristems. In addition, we cloned a PCR fragment corresponding to the DNA binding domain of human Cdc5-like protein. These results strongly suggest that Cdc5-like protein exists in all eukaryotes and may function in cell cycle regulation.

Original languageEnglish
Pages (from-to)13371-13376
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number23
DOIs
Publication statusPublished - Nov 12 1996
Externally publishedYes

Fingerprint

Arabidopsis
Schizosaccharomyces
Complementary DNA
Proteins
Genes
Meristem
Eukaryota
Recombinant Proteins
Amino Acid Sequence
Cell Cycle
Phenotype
Amino Acids
Polymerase Chain Reaction
Peptides
Temperature
DNA
Growth

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A cdc5+ homolog of a higher plant, Arabidopsis thaliana. / Hirayama, Takashi; Shinozaki, Kazuo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 23, 12.11.1996, p. 13371-13376.

Research output: Contribution to journalArticle

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