A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1

Tomoyuki Nakagawa, Ryoji Mitsui, Akio Tani, Kentaro Sasa, Shinya Tashiro, Tomonori Iwama, Takashi Hayakawa, Keiichi Kawai

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Abstract

In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1.

Original languageEnglish
Article numbere50480
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 27 2012

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Methylobacterium extorquens
Methanol
methanol
Metabolism
Genes
Enzymes
calcium
alcohol dehydrogenase (acceptor)
Amino Acid Sequence
Bacteria
Genome
Amino Acids
metabolism
Oxidation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1. / Nakagawa, Tomoyuki; Mitsui, Ryoji; Tani, Akio; Sasa, Kentaro; Tashiro, Shinya; Iwama, Tomonori; Hayakawa, Takashi; Kawai, Keiichi.

In: PLoS One, Vol. 7, No. 11, e50480, 27.11.2012.

Research output: Contribution to journalArticle

Nakagawa, T, Mitsui, R, Tani, A, Sasa, K, Tashiro, S, Iwama, T, Hayakawa, T & Kawai, K 2012, 'A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1', PLoS One, vol. 7, no. 11, e50480. https://doi.org/10.1371/journal.pone.0050480
Nakagawa, Tomoyuki ; Mitsui, Ryoji ; Tani, Akio ; Sasa, Kentaro ; Tashiro, Shinya ; Iwama, Tomonori ; Hayakawa, Takashi ; Kawai, Keiichi. / A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1. In: PLoS One. 2012 ; Vol. 7, No. 11.
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