Abstract
Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.
| Original language | English |
|---|---|
| Journal | Journal of biological rhythms |
| DOIs | |
| Publication status | Accepted/In press - Jan 1 2019 |
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Keywords
- Drosophila
- clock neuron
- split-GAL4
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster. / Sekiguchi, Manabu; Inoue, Kotaro; Yang, Tian; Luo, Dong Gen; Yoshii, Taishi.
In: Journal of biological rhythms, 01.01.2019.Research output: Contribution to journal › Letter
}
TY - JOUR
T1 - A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster
AU - Sekiguchi, Manabu
AU - Inoue, Kotaro
AU - Yang, Tian
AU - Luo, Dong Gen
AU - Yoshii, Taishi
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.
AB - Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.
KW - Drosophila
KW - clock neuron
KW - split-GAL4
UR - http://www.scopus.com/inward/record.url?scp=85077146253&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077146253&partnerID=8YFLogxK
U2 - 10.1177/0748730419895154
DO - 10.1177/0748730419895154
M3 - Letter
AN - SCOPUS:85077146253
JO - Journal of Biological Rhythms
JF - Journal of Biological Rhythms
SN - 0748-7304
ER -