TY - JOUR
T1 - A Catalog of GAL4 Drivers for Labeling and Manipulating Circadian Clock Neurons in Drosophila melanogaster
AU - Sekiguchi, Manabu
AU - Inoue, Kotaro
AU - Yang, Tian
AU - Luo, Dong Gen
AU - Yoshii, Taishi
N1 - Funding Information:
Drosophila melanogaster Sekiguchi Manabu * 1 Inoue Kotaro † 1 Yang Tian ‡ § || ¶ Luo Dong-Gen ‡ § || ¶ https://orcid.org/0000-0002-7057-7986 Yoshii Taishi † 2 * Matching Program Course, Okayama University, Okayama, Japan † Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan ‡ PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, China § Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China || College of Life Sciences, Peking University, Beijing, China ¶ Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China 2 Taishi Yoshii, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka 3-1, Kita-ku, Okayama, Okayama 700-8530, Japan; e-mail: yoshii@okayama-u.ac.jp . 1 These authors contributed equally to this work. 12 2019 0748730419895154 © 2019 The Author(s) 2019 SAGE Publications Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila , the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock. clock neuron split-GAL4 Drosophila Japan Society for the Promotion of Science https://doi.org/10.13039/501100001691 KAKENHI 15H05600 Japan Society for the Promotion of Science https://doi.org/10.13039/501100001691 KAKENHI 19H03265 national natural science foundation of china https://doi.org/10.13039/501100001809 31671085, 31871058 and 31930043 edited-state corrected-proof typesetter ts1 This work was funded by the JSPS (KAKENHI 15H05600 and 19H03265) and National Natural Science Foundation of China (31671085, 31871058 and 31930043). We would like to thank C. Hermann-Luibl for preliminary work and comments on the article and the Janelia Flylight project team, the Bloomington Drosophila Stock Center, and the Vienna Drosophila Resource Center for providing fly lines. We are also grateful to J. Blau and the Developmental Studies Hybridoma Bank for providing antibodies. Conflict Of Interest Statement The authors have no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. ORCID iD Taishi Yoshii https://orcid.org/0000-0002-7057-7986
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.
AB - Daily rhythms of physiology, metabolism, and behavior are orchestrated by a central circadian clock. In mice, this clock is coordinated by the suprachiasmatic nucleus, which consists of 20,000 neurons, making it challenging to characterize individual neurons. In Drosophila, the clock is controlled by only 150 clock neurons that distribute across the fly’s brain. Here, we describe a comprehensive set of genetic drivers to facilitate individual characterization of Drosophila clock neurons. We screened GAL4 lines that were obtained from Drosophila stock centers and identified 63 lines that exhibit expression in subsets of central clock neurons. Furthermore, we generated split-GAL4 lines that exhibit specific expression in subsets of clock neurons such as the 2 DN2 neurons and the 6 LPN neurons. Together with existing driver lines, these newly identified ones are versatile tools that will facilitate a better understanding of the Drosophila central circadian clock.
KW - Drosophila
KW - clock neuron
KW - split-GAL4
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U2 - 10.1177/0748730419895154
DO - 10.1177/0748730419895154
M3 - Letter
AN - SCOPUS:85077146253
VL - 35
SP - 207
EP - 213
JO - Journal of Biological Rhythms
JF - Journal of Biological Rhythms
SN - 0748-7304
IS - 2
ER -