A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans

Yoshishige Kimura, Ethan E. Corcoran, Koh Eto, Keiko Gengyo-Ando, Masa Aki Muramatsu, Ryoji Kobayashi, Jonathan H. Freedman, Shohei Mitani, Masatoshi Hagiwara, Anthony R. Means, Hiroshi Tokumitsu

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes.

Original languageEnglish
Pages (from-to)962-966
Number of pages5
JournalEMBO Reports
Volume3
Issue number10
DOIs
Publication statusPublished - Oct 1 2002
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Transcription
Neurons
Cyclic AMP Response Element-Binding Protein
Phosphotransferases
Calmodulin
Calcium-Calmodulin-Dependent Protein Kinase Type 1
Calcium-Calmodulin-Dependent Protein Kinase Type 4
Calcium-Calmodulin-Dependent Protein Kinase Kinase
Head
Neuronal Plasticity
Mammals
Enzyme activity
Muscle Contraction
Gene expression
Plasticity
Cell Proliferation
Calcium
Sensors
Enzymes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kimura, Y., Corcoran, E. E., Eto, K., Gengyo-Ando, K., Muramatsu, M. A., Kobayashi, R., ... Tokumitsu, H. (2002). A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. EMBO Reports, 3(10), 962-966. https://doi.org/10.1093/embo-reports/kvf191

A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. / Kimura, Yoshishige; Corcoran, Ethan E.; Eto, Koh; Gengyo-Ando, Keiko; Muramatsu, Masa Aki; Kobayashi, Ryoji; Freedman, Jonathan H.; Mitani, Shohei; Hagiwara, Masatoshi; Means, Anthony R.; Tokumitsu, Hiroshi.

In: EMBO Reports, Vol. 3, No. 10, 01.10.2002, p. 962-966.

Research output: Contribution to journalArticle

Kimura, Y, Corcoran, EE, Eto, K, Gengyo-Ando, K, Muramatsu, MA, Kobayashi, R, Freedman, JH, Mitani, S, Hagiwara, M, Means, AR & Tokumitsu, H 2002, 'A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans', EMBO Reports, vol. 3, no. 10, pp. 962-966. https://doi.org/10.1093/embo-reports/kvf191
Kimura Y, Corcoran EE, Eto K, Gengyo-Ando K, Muramatsu MA, Kobayashi R et al. A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. EMBO Reports. 2002 Oct 1;3(10):962-966. https://doi.org/10.1093/embo-reports/kvf191
Kimura, Yoshishige ; Corcoran, Ethan E. ; Eto, Koh ; Gengyo-Ando, Keiko ; Muramatsu, Masa Aki ; Kobayashi, Ryoji ; Freedman, Jonathan H. ; Mitani, Shohei ; Hagiwara, Masatoshi ; Means, Anthony R. ; Tokumitsu, Hiroshi. / A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. In: EMBO Reports. 2002 ; Vol. 3, No. 10. pp. 962-966.
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