A blinded randomized assessment of laser Doppler flowmetry efficacy in standardizing outcome from intraluminal filament MCAO in the rat

Hideki Taninishi, Jin Yong Jung, Miwa Izutsu, Zhengfeng Wang, Huaxin Sheng, David S. Warner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Laser Doppler flowmetry (LDF) is widely used for estimating cerebral blood flow changes during intraluminal middle cerebral artery occlusion (MCAO). No investigation has systematically examined LDF efficacy in standardizing outcome. We examined MCAO histologic and behavioral outcome as a function of LDF measurement. Materials and methods: Rats were subjected to 90. min MCAO by 4 surgeons having different levels of MCAO surgical experience. LDF was measured in all rats during ischemia. By random assignment, LDF values were (Assisted) or were not (Blinded) made available to each surgeon during MCAO (n= 12-17 per group). Neurologic and histologic outcomes were measured 7 days post-MCAO. A second study examined LDF effects on 1-day post-MCAO outcome. Results: Pooled across surgeons, intra-ischemic %LDF change (P=0.12), neurologic scores (Assisted vs. Blinded=14±6 vs. 13±7, P=0.61, mean±standard deviation) and cerebral infarct volume (162±63mm3 vs. 143±86mm3, P=0.24) were not different between groups. Only for one surgeon (novice) did LDF use alter infarct volume (145±28mm3 vs. 98±61mm3, P=0.03). LDF use decreased infarct volume coefficient of variation (COV) by 35% (P=0.02), but had no effect on neurologic score COV. Comparison with existing methods: We compared intraluminal MCAO outcome as a function of LDF use. Conclusions: LDF measurement altered neither neurologic nor histologic MCAO outcome. LDF did not decrease neurologic deficit COV, but did decrease infarct volume COV. LDF may allow use of fewer animals if infarct volume is the primary dependent variable, but is unlikely to impact requisite sample sizes if neurologic function is of primary interest.

Original languageEnglish
Pages (from-to)111-120
Number of pages10
JournalJournal of Neuroscience Methods
Volume241
DOIs
Publication statusPublished - Feb 5 2015
Externally publishedYes

Keywords

  • Cerebral blood flow
  • Intraluminal
  • Laser Doppler flowmetry
  • Middle cerebral artery occlusion
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)

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