A Biodegradable Multifunctional Graphene Oxide Platform for Targeted Cancer Therapy

Cristina Martín, Amalia Ruiz, Sandeep Keshavan, Giacomo Reina, Diane Murera, Yuta Nishina, Bengt Fadeel, Alberto Bianco

Research output: Contribution to journalArticle

Abstract

The design of multifunctional materials able to both selectively deliver a drug into cells in a targeted manner and display an enhanced propensity for biodegradation is an important goal. Here, graphene oxide (GO) is functionalized with the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) known to interact with the formyl peptide receptor, which is expressed in different cancer cells, including cervical carcinoma cells. This study highlights the ability of GOfMLP for targeted drug delivery and cancer cell killing and the subsequent degradation capacity of the hybrid. Biodegradation is assessed via Raman spectroscopy and transmission electron microscopy. The results show that GOfMLP is susceptible to faster myeloperoxidase-mediated degradation. The hybrid material, but not GO, is capable of inducing neutrophil degranulation with subsequent degradation, being the first study showing inducible neutrophil degradation by the nanomaterial itself with no prior activation of the cells. In addition, confocal imaging and flow cytometry using HeLa cells demonstrate that GOfMLP is able to deliver the chemotherapeutic agent doxorubicin faster into cells, inducing higher levels of apoptosis, when compared to nonfunctionalized GO. The results reveal that GOfMLP is a promising carrier able to efficiently deliver anticancer drugs, being endowed with the ability to induce its own biodegradation.

Original languageEnglish
Article number1901761
JournalAdvanced Functional Materials
Volume29
Issue number39
DOIs
Publication statusPublished - Sep 1 2019

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Graphite
biodegradation
Oxides
Graphene
therapy
graphene
drugs
platforms
Biodegradation
cancer
degradation
neutrophils
Degradation
oxides
Cells
cells
Peptides
peptides
Formyl Peptide Receptor
N-Formylmethionine Leucyl-Phenylalanine

Keywords

  • biodegradation
  • cancer
  • chemoattractants
  • drug delivery
  • graphene

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

A Biodegradable Multifunctional Graphene Oxide Platform for Targeted Cancer Therapy. / Martín, Cristina; Ruiz, Amalia; Keshavan, Sandeep; Reina, Giacomo; Murera, Diane; Nishina, Yuta; Fadeel, Bengt; Bianco, Alberto.

In: Advanced Functional Materials, Vol. 29, No. 39, 1901761, 01.09.2019.

Research output: Contribution to journalArticle

Martín, C, Ruiz, A, Keshavan, S, Reina, G, Murera, D, Nishina, Y, Fadeel, B & Bianco, A 2019, 'A Biodegradable Multifunctional Graphene Oxide Platform for Targeted Cancer Therapy', Advanced Functional Materials, vol. 29, no. 39, 1901761. https://doi.org/10.1002/adfm.201901761
Martín, Cristina ; Ruiz, Amalia ; Keshavan, Sandeep ; Reina, Giacomo ; Murera, Diane ; Nishina, Yuta ; Fadeel, Bengt ; Bianco, Alberto. / A Biodegradable Multifunctional Graphene Oxide Platform for Targeted Cancer Therapy. In: Advanced Functional Materials. 2019 ; Vol. 29, No. 39.
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