A binding protein for fungal signal molecules in the cell wall of Pisum sativum

Akinori Kiba, Takako Ohgawara, Kazuhiro Toyoda, Miho Inoue-Ozaki, Tadahiro Takeda, Uppalapati Srinivasa Rao, Toshiaki Kato, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous signal molecules.

Original languageEnglish
Pages (from-to)228-237
Number of pages10
JournalJournal of General Plant Pathology
Volume72
Issue number4
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Pisum sativum
adenosinetriphosphatase
binding proteins
Mycosphaerella pinodes
cell walls
apyrase
peas
peroxidases
anion exchange
solubilization
ammonium sulfate
superoxide anion
agarose
chromatography
peroxidase
Western blotting
amino acid sequences
proteins
clones
seedlings

Keywords

  • Binding protein for fungal signals
  • Cell-wall-bound ATPase (NTPase)
  • Elicitor
  • Mycosphaerella pinodes
  • Pisum sativum L.
  • Suppressor of defense

ASJC Scopus subject areas

  • Plant Science

Cite this

Kiba, A., Ohgawara, T., Toyoda, K., Inoue-Ozaki, M., Takeda, T., Rao, U. S., ... Shiraishi, T. (2006). A binding protein for fungal signal molecules in the cell wall of Pisum sativum. Journal of General Plant Pathology, 72(4), 228-237. https://doi.org/10.1007/s10327-006-0278-8

A binding protein for fungal signal molecules in the cell wall of Pisum sativum. / Kiba, Akinori; Ohgawara, Takako; Toyoda, Kazuhiro; Inoue-Ozaki, Miho; Takeda, Tadahiro; Rao, Uppalapati Srinivasa; Kato, Toshiaki; Ichinose, Yuki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 72, No. 4, 08.2006, p. 228-237.

Research output: Contribution to journalArticle

Kiba, A, Ohgawara, T, Toyoda, K, Inoue-Ozaki, M, Takeda, T, Rao, US, Kato, T, Ichinose, Y & Shiraishi, T 2006, 'A binding protein for fungal signal molecules in the cell wall of Pisum sativum', Journal of General Plant Pathology, vol. 72, no. 4, pp. 228-237. https://doi.org/10.1007/s10327-006-0278-8
Kiba, Akinori ; Ohgawara, Takako ; Toyoda, Kazuhiro ; Inoue-Ozaki, Miho ; Takeda, Tadahiro ; Rao, Uppalapati Srinivasa ; Kato, Toshiaki ; Ichinose, Yuki ; Shiraishi, Tomonori. / A binding protein for fungal signal molecules in the cell wall of Pisum sativum. In: Journal of General Plant Pathology. 2006 ; Vol. 72, No. 4. pp. 228-237.
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