A bifurcated palea mutant infers functional differentiation of WOX3 genes in flower and leaf morphogenesis of barley

Takanori Yoshikawa, Hiroshi Hisano, Ken Ichiro Hibara, Jilu Nie, Yuki Tanaka, Jun Ichi Itoh, Shin Taketa

Research output: Contribution to journalArticlepeer-review

Abstract

Barley (Hordeum vulgare) is the fourth most highly produced cereal in the world after wheat, rice and maize and is mainly utilized as malts and for animal feed. Barley, a model crop of the tribe Triticeae, is important in comparative analyses of Poaceae. However, molecular understanding about the developmental processes is limited in barley. Our previous work characterized one of two WUSCHEL-RELATED HOMEOBOX 3 (WOX3) genes present in the barley genome: NARROW LEAFED DWARF1 (NLD1). We demonstrated that NLD1 plays a pivotal role in the development of lateral organs. In the present study, we describe a bifurcated palea (bip) mutant of barley focusing on flower and leaf phenotypes. The palea in the bip mutant was split into two and develop towards inside the lemma surrounding the carpels and anthers. The bip mutant is devoid of lodicules, which develop in a pair at the base of the stamen within the lemma in normal barley. bip also exhibited malformations in leaves, such as narrow leaf due to underdeveloped leaf-blade width, and reduced trichome density. Map-based cloning and expression analysis indicated that BIP is identical to another barley WOX3 gene, named HvWOX3. The bip nld1 double mutant presented a more severe reduction in leaf-blade width and number of trichomes. By comparing the phenotypes and gene expression patterns of various WOX3 mutants, we concluded that leaf bilateral outgrowth and trichome development are promoted by both NLD1 and HvWOX3, but that HvWOX3 serves unique and pivotal functions in barley development that differ from those of NLD1.

Original languageEnglish
Article numberplac019
JournalAoB PLANTS
Volume14
Issue number3
DOIs
Publication statusPublished - Jun 1 2022

Keywords

  • Barley
  • floral organ
  • Hordeum vulgare
  • leaf
  • trichome
  • WUSCHEL-RELATED HOMEOBOX 3

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'A bifurcated palea mutant infers functional differentiation of WOX3 genes in flower and leaf morphogenesis of barley'. Together they form a unique fingerprint.

Cite this