A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)

Sho Ohno, Munetaka Hosokawa, Atsushi Hoshino, Yoshikuni Kitamura, Yasumasa Morita, Kyeung Ii Park, Akiko Nakashima, Ayumi Deguchi, Fumi Tatsuzawa, Motoaki Doi, Shigeru Iida, Susumu Yazawa

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Dahlias (Dahlia variabilis) exhibit a wide range of flower colours because of accumulation of anthocyanin and other flavonoids in their ray florets. Two lateral mutants were used that spontaneously occurred in 'Michael J' (MJW) which has yellow ray florets with orange variegation. MJOr, a bud mutant producing completely orange ray florets, accumulates anthocyanins, flavones, and butein, and MJY, another mutant producing completely yellow ray florets, accumulates flavones and butein. Reverse transcription-PCR analysis showed that expression of chalcone synthase 1 (DvCHS1), flavanone 3-hydroxylase (DvF3H), dihydroflavonol 4-reductase (DvDFR), anthocyanidin synthase (DvANS), and DvIVS encoding a basic helix-loop-helix transcription factor were suppressed, whereas that of chalcone isomerase (DvCHI) and DvCHS2, another CHS with 69% nucleotide identity with DvCHS1, was not suppressed in the yellow ray florets of MJY. A 5.4kb CACTA superfamily transposable element, transposable element of Dahlia variabilis 1 (Tdv1), was found in the fourth intron of the DvIVS gene of MJW and MJY, and footprints of Tdv1 were detected in the variegated flowers of MJW. It is shown that only one type of DvIVS gene was expressed in MJOr, whereas these plants are likely to have three types of the DvIVS gene. On the basis of these results, the mechanism regulating the formation of orange and yellow ray florets in dahlia is discussed.

Original languageEnglish
Pages (from-to)5105-5116
Number of pages12
JournalJournal of experimental botany
Volume62
Issue number14
DOIs
Publication statusPublished - Oct 2011

Keywords

  • Anthocyanin
  • CACTA superfamily
  • bHLH
  • dahlia
  • transcription factor

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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